Slit1 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NB600-880
Key Product Details
Species Reactivity
Mouse, Rat
Applications
ELISA, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This affinity purified Slit1 Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near aa 485-515 of mouse Slit1 protein. (Uniprot: Q80TR4)
Reactivity Notes
A BLAST analysis was used to suggest reactivity with this protein from mouse and rat based on 100% homology for the immunogen sequence. Expect cross reactivity with SLIT-1 from human, chicken and frog sources, as only a single amino acid residue change is found within the immunogen sequence (94% positive by BLAST). Cross reactivity with SLIT-1 homologues from other sources has not been determined.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
This affinity purified antibody is directed against mouse Slit1 protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification
Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
Scientific Data Images for Slit1 Antibody
![Western Blot: Slit1 Antibody [NB600-880]](https://resources.bio-techne.com/images/products/Slit1-Antibody-Western-Blot-NB600-880-img0001.jpg "Western Blot: Slit1 Antibody [NB600-880]")
Western Blot: Slit1 Antibody [NB600-880]
Western Blot: Slit1 Antibody [NB600-880] - Analysis of SLIT-1 in rat (lane 1) and mouse (lane 2) brain lysates. The expected molecular weight for SLIT-1 is 168 kDa. Approximately 20 ug of each lysates was run on a SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1:500 dilution of anti-SLIT-1 antibody. Signal was detected using standard techniques. Note: The smaller strong bands observed in this blot are likely SLIT-1 cleavage products. A number of cleavage products for both Slit1 and Slit2 are reported in the literature resulting from alternate splicing and range from 40kDa -160kDa (see Little et al, 2002 for additional details).Applications for Slit1 Antibody
Application
Recommended Usage
ELISA
1:10000-1:50000
Western Blot
1:500-1:2000
Application Notes
This affinity purified antibody has been tested for use in ELISA and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect multiple bands corresponding to cleavage products of SLIT-1 by western blotting in the appropriate cell lysate or extract.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.
Background: Slit1
Long Name
SLIT Homolog 1
Alternate Names
MEGF4, SLIL1
Gene Symbol
SLIT1
UniProt
Additional Slit1 Products
Product Documents for Slit1 Antibody
Product Specific Notices for Slit1 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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