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Slit3 Antibody (3C5)

Novus Biologicals, part of Bio-Techne | Catalog # H00006586-M04

Novus Biologicals, part of Bio-Techne
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H00006586-M04

Key Product Details

Species Reactivity

Human

Applications

ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 3C5

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

SLIT3 (NP_003053, 1371 a.a. ~ 1470 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. PCLGHRCHHGKCVATGTSYMCKCAEGYGGDLCDNKNDSANACSAFKCHHGQCHISDQGEPYCLCQPGFSGEHCQQENPCLGQVVREVIRRQKGYASCATA

Localization

Secreted

Specificity

SLIT3 - slit homolog 3 (Drosophila)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for Slit3 Antibody (3C5)

ELISA: Slit3 Antibody (3C5) [H00006586-M04]

ELISA: Slit3 Antibody (3C5) [H00006586-M04]

ELISA: Slit3 Antibody (3C5) [H00006586-M04] - Detection limit for recombinant GST tagged SLIT3 is approximately 0.03ng/ml as a capture antibody.

Applications for Slit3 Antibody (3C5)

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB. GST tag alone is used as a negative control.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Slit3

Slit3 (also known as multiple epidermal growth factor like domain 5 and Slit homolog 3 protein) is a Slit protein. The 'slit' gene has been shown to play a critical role in central nervous system midline formation. In addition to Slit3 there are two additional human 'slit' homologs, which are termed Slit1 and Slit2. Each SLIT gene encodes a putative secreted protein, which contains conserved protein-protein interaction domains including leucine-rich repeats and epidermal growth factor-like motifs, similar to those of the Drosophila protein. SLIT proteins may also participate in the formation and maintenance of the nervous and endocrine systems by protein-protein interactions. Slit3 is a secreted protein predominantly expressed in thyroid. Three isoforms have been reported for this product.

Long Name

SLIT Homolog 3

Alternate Names

MEGF5, SLIL2

Entrez Gene IDs

6586 (Human)

Gene Symbol

SLIT3

OMIM

603745 (Human)

UniProt

Additional Slit3 Products

Product Documents for Slit3 Antibody (3C5)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Slit3 Antibody (3C5)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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