Smad1 Antibody (7H7L5)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15670

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-15670-100ul
NBP3-15670-20ul
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Key Product Details

Validated by

Knockout/Knockdown, Biological Validation

Species Reactivity

Validated:

Human, Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Knockout Validated, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 7H7L5

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Smad1 Antibodies »

Product Summary for Smad1 Antibody (7H7L5)

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 20-240 of human Smad1 (NP_005891.1). WKQGDEEEKWAEKAVDALVKKLKKKKGAMEELEKALSCPGQPSNCVTIPRSLDGRLQVSHRKGLPHVIYCRVWRWPDLQSHHELKPLECCEFPFGSKQKEVCINPYHYKRVESPVLPPVLVPRHSEYNPQHSLLAQFRNLGQNEPHMPLNATFPDSFQQPNSHPFPHSPNSSYPNSPGSSSSTYPHSPTSSDPGSPFQMPADTPPPAYLPPEDPMTQDGSQ

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Smad1 Antibody (7H7L5)

Western Blot: Smad1 Antibody (7H7L5) [NBP3-15670]

Western Blot: Smad1 Antibody (7H7L5) [NBP3-15670]

Western Blot: Smad1 Antibody (4W2M9) [NBP3-15670] - Western blot analysis of extracts of HUVEC cells, using Smad1 antibody (NBP3-15670) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
Immunocytochemistry/ Immunofluorescence: Smad1 Antibody (7H7L5) [NBP3-15670]

Immunocytochemistry/ Immunofluorescence: Smad1 Antibody (7H7L5) [NBP3-15670]

Immunocytochemistry/Immunofluorescence: Smad1 Antibody (4W2M9) [NBP3-15670] - Immunofluorescence analysis of NIH-3T3 cells using [KO Validated] Smad1 Rabbit mAb (NBP3-15670) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunohistochemistry-Paraffin: Smad1 Antibody (7H7L5) [NBP3-15670]

Immunohistochemistry-Paraffin: Smad1 Antibody (7H7L5) [NBP3-15670]

Immunohistochemistry-Paraffin: Smad1 Antibody (4W2M9) [NBP3-15670] - Immunohistochemistry of paraffin-embedded human colon carcinoma using Smad1 antibody (NBP3-15670) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
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Applications for Smad1 Antibody (7H7L5)

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Immunoprecipitation

1:500 - 1:1000

Western Blot

1:500 - 1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.05% Proclin 300

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Smad1

Smad1 is encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein mediates the signals of the bone morphogenetic proteins (BMPs), which are involved in a range of biological activities including cell growth, apoptosis, morphogenesis, development and immune responses. In response to BMP ligands, this protein can be phosphorylated and activated by the BMP receptor kinase. The phosphorylated form of this protein forms a complex with SMAD4, which is important for its function in the transcription regulation. This protein is a target for SMAD-specific E3 ubiquitin ligases, such as SMURF1 and SMURF2, and undergoes ubiquitination and proteasome-mediated degradation. Alternatively spliced transcript variants encoding the same protein have been observed.

Long Name

Mothers Against DPP Homolog 1

Alternate Names

BSP1, BSP-1, hSMAD1, JV4-1SMAD, mothers against DPP homolog 1 (Drosophila), MAD homolog 1, MADH1JV41, MADR1MAD, mothers against decapentaplegic homolog 1 (Drosophila), Mad-related protein 1, mothers against decapentaplegic homolog 1, Mothers against DPP homolog 1, SMAD family member 1SMAD 1, SMAD, mothers against DPP homolog 1, Smad1, TGF-beta signaling protein 1, transforming growth factor-beta signaling protein 1

Gene Symbol

SMAD1

Additional Smad1 Products

Product Documents for Smad1 Antibody (7H7L5)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Smad1 Antibody (7H7L5)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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