Smad2 [p Ser255] Antibody (2A12)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-26391

Recombinant monoclonal antibody expressed in HEK293F cells
Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 2A12

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Smad2 Antibodies »

Product Summary for Smad2 [p Ser255] Antibody (2A12)

Immunogen

A synthesized peptide derived from Human SMAD2 [p Ser255] [UniProt Q15796]

Modification

p Ser 255

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Smad2 [p Ser255] Antibody (2A12)

Smad2 [p Ser255] Antibody (2A12)

Immunocytochemistry/Immunofluorescence: Smad2 [p Ser255] Antibody (2A12) [NBP3-26391] -

Immunocytochemistry/Immunofluorescence: Smad2 [p Ser255] Antibody (2A12) [NBP3-26391] - Staining of A549 cells with Smad2 [p Ser255] Antibody (2A12) at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG (H+L).

Applications for Smad2 [p Ser255] Antibody (2A12)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:20-1:200
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, pH 7.4, 150mM NaCl and 50% glycerol

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -70C. Avoid freeze-thaw cycles.

Background: Smad2

Smad 2 is a member of the Mothers Against Dpp (MAD)-related family of proteins. So far, nine Smads have been identified and can be divided in 3 subgroups based on their structure and functions; pathway-restricted, common mediator and inhibitory Smad. Smad 2 and 3 serve as pathway-restricted Smads for the TGF-beta/activin signaling pathways (1-2). Smad 2 is phosphorylated by the TGF-beta type 1 receptor kinase on Ser 465 and Ser 467. Phosphorylation of both serines is essential for Smad 2-Smad4 heteromeric complex and dissociation of Smad 2 from the TGF-beta type 1 receptor (3). Once phosphorylated Smad2 translocate to the nucleus where it associates with DNA-binding proteins and forming a transcriptional complex (4). Unlike Smad 3 and 4, Smad 2 does not directly bind DNA (5).

Long Name

Mothers Against DPP Homolog 2

Alternate Names

JV18-1, MADH2, MADR2, Smox

Gene Symbol

SMAD2

Additional Smad2 Products

Product Documents for Smad2 [p Ser255] Antibody (2A12)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Smad2 [p Ser255] Antibody (2A12)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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