Smad2 Antibody (CL8472)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-88916

Novus Biologicals, part of Bio-Techne
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NBP2-88916
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Predicted:

Mouse (94%), Rat (94%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # CL8472

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Smad2 Antibodies »

Product Summary for Smad2 Antibody (CL8472)

Immunogen

This antibody was developed against a synthetic peptide corresponding to amino acids: PNTIDQWDTTGLYSFSEQT

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Smad2 Antibody (CL8472)

Immunohistochemistry-Paraffin: Smad2 Antibody (CL8472) [NBP2-88916]

Immunohistochemistry-Paraffin: Smad2 Antibody (CL8472) [NBP2-88916]

Immunohistochemistry-Paraffin: Smad2 Antibody (CL8472) [NBP2-88916] - Analysis in human fallopian tube and pancreas tissues using NBP2-88916 antibody. Corresponding SMAD2 RNA-seq data are presented for the same tissues.
Western Blot: Smad2 Antibody (CL8472) [NBP2-88916]

Western Blot: Smad2 Antibody (CL8472) [NBP2-88916]

Western Blot: Smad2 Antibody (CL8472) [NBP2-88916] - Analysis in human cell line U-138MG.
Immunocytochemistry/ Immunofluorescence: Smad2 Antibody (CL8472) [NBP2-88916]

Immunocytochemistry/ Immunofluorescence: Smad2 Antibody (CL8472) [NBP2-88916]

Immunocytochemistry/Immunofluorescence: Smad2 Antibody (CL8472) [NBP2-88916] - Staining of HeLa cells using the Anti-SMAD2 monoclonal antibody, showing specific staining in the cytosol and nucleoplasm in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).
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Applications for Smad2 Antibody (CL8472)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

1 ug/ml
Application Notes
For ICC/IF, PFA/Triton X-100 is recommended for fixation/permeabilization. For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS, pH 7.2, containing 40% glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Smad2

Smad 2 is a member of the Mothers Against Dpp (MAD)-related family of proteins. So far, nine Smads have been identified and can be divided in 3 subgroups based on their structure and functions; pathway-restricted, common mediator and inhibitory Smad. Smad 2 and 3 serve as pathway-restricted Smads for the TGF-beta/activin signaling pathways (1-2). Smad 2 is phosphorylated by the TGF-beta type 1 receptor kinase on Ser 465 and Ser 467. Phosphorylation of both serines is essential for Smad 2-Smad4 heteromeric complex and dissociation of Smad 2 from the TGF-beta type 1 receptor (3). Once phosphorylated Smad2 translocate to the nucleus where it associates with DNA-binding proteins and forming a transcriptional complex (4). Unlike Smad 3 and 4, Smad 2 does not directly bind DNA (5).

Long Name

Mothers Against DPP Homolog 2

Alternate Names

JV18-1, MADH2, MADR2, Smox

Gene Symbol

SMAD2

Additional Smad2 Products

Product Documents for Smad2 Antibody (CL8472)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Smad2 Antibody (CL8472)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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