Smad2 [p Thr220] Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-04982

Novus Biologicals, part of Bio-Techne
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NBP1-04982
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Mouse

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all Smad2 Antibodies »

Product Specifications

Immunogen

Peptide sequence around phosphorylation site of threonine 220 (P-E-T(p)-P-P) derived from Human Smad2 (NP_001003652.1).

Modification

p Thr220

Specificity

Detects endogenous levels of Smad2 only when phosphorylated at threonine 220.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

52 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Smad2 [p Thr220] Antibody

Western Blot: Smad2 [p Thr220] Antibody [NBP1-04982]

Western Blot: Smad2 [p Thr220] Antibody [NBP1-04982]

Western Blot: Smad2 [p Thr220] Antibody [NBP1-04982] - Extract from Hela cells untreated or treated with IL-4(100ng/mL, 30min), using Smad2 (Ab-220) Antibody (NBP1-04981, Lanes 1 and 2) and Smad2 (Phospho-Thr220) Antibody (NBP1-04982, Lanes 3 and 4).
Western Blot: Smad2 [p Thr220] Antibody [NBP1-04982]

Western Blot: Smad2 [p Thr220] Antibody [NBP1-04982]

Western Blot: Smad2 [p Thr220] Antibody [NBP1-04982] - Extracts from Hela cells untreated or treeated with PMAA using Smadd2 (Phospho-Thhr220) Antibody .
Smad2 [p Thr220] Antibody

Immunohistochemistry-Paraffin: Smad2 [p Thr220] Antibody [NBP1-04982] -

Immunohistochemistry-Paraffin: Smad2 [p Thr220] Antibody [NBP1-04982] - Immunohistochemical analysis of Smad2 staining in human brain formalin fixed paraffin embedded tissue section.
View 2 more images

Applications for Smad2 [p Thr220] Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:500

Immunohistochemistry

1:50-1:200

Immunohistochemistry-Paraffin

1:10-1:500

Western Blot

1:500-1:1000
Application Notes
IHC-P reactivity reported in scientific literature (PMID: 23370707).
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol

Preservative

0.01% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Smad2

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Long Name

Mothers Against DPP Homolog 2

Alternate Names

JV18-1, MADH2, MADR2, Smox, pSMAD

Entrez Gene IDs

4087 (Human); 17126 (Mouse); 29357 (Rat)

Gene Symbol

SMAD2

UniProt

Q15796 (Human)

Additional Smad2 Products

Product Documents for Smad2 [p Thr220] Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Smad2 [p Thr220] Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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