Smad4 Antibody (BLR133J)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-14714

Recombinant Monoclonal
Novus Biologicals, part of Bio-Techne
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NBP3-14714
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Key Product Details

Species Reactivity

Human, Mouse

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit Clone # BLR133J

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Smad4 Antibodies »

Product Specifications

Immunogen

between 1 and 50

Clonality

Monoclonal

Host

Rabbit

Scientific Data Images for Smad4 Antibody (BLR133J)

Immunoprecipitation: Smad4 Antibody (BLR133J) [NBP3-14714]

Immunoprecipitation: Smad4 Antibody (BLR133J) [NBP3-14714]

Immunoprecipitation: Smad4 Antibody [NBP3-14714] - : Whole cell lysate (1.0 mgper IP reaction; 20% of IP loaded) from 293T cells preparedusing NETN lysis buffer. Antibodies: Rabbit anti-SMAD4recombinant monoclonal antibody used for IP at 6 ul per reaction. SMAD4 was alsoimmunoprecipitated by rabbit anti-SMAD4 antibody. For blotting immunoprecipitated SMAD4, was used at 1:1000. Chemiluminescence withan exposure time of 3 seconds.

Applications for Smad4 Antibody (BLR133J)

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

>95%

Formulation

PBS, 2% Sucrose

Preservative

0.09% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 2 - 8 C / 1 year from date of receipt

Background: Smad4

Smad proteins, the mammalian homologs of the Drosophila Mothers against dpp (Mad) have been implicated as downstream effectors of TGFbeta/BMP signaling. Smad1 (also designated Madr1 or JV4-1), Smad5 and mammalian Smad8 (also designated Smad9 or MadH6) are effectors of BMP2 and BMP4 function while Smad2 (also designated Madr2 or JV18-1) and Smad3 are involved in TGFbeta and activin-mediated growth modulation. Smad4 (also designated DPC4) has been shown to mediate all of the above activities through interaction with various Smad family members. Smad6 and Smad7 regulate the response to activin/TGFbeta signaling by interfering with TGFbeta-mediated phosphorylation of other Smad family members.

Long Name

Mothers Against DPP Homolog 4

Alternate Names

DPC4, MADH4

Gene Symbol

SMAD4

Additional Smad4 Products

Product Documents for Smad4 Antibody (BLR133J)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Smad4 Antibody (BLR133J)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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