Smad7 Antibody - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-94047

Novus Biologicals, part of Bio-Techne
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NBP2-94047-0.1ml
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Smad7 Antibodies »

Product Summary for Smad7 Antibody - Azide and BSA Free

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Smad7 (NP_005895.1). MFRTKRSALVRRLWRSRAPGGEDEEEGAGGGGGGGELRGEGATDSRAHGAGGGGPGRAGCCLGKAVRGAKGHHHPHPPAAGAGAAGGAEADLKALTHSVL

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Smad7 Antibody - Azide and BSA Free

Western Blot: Smad7 AntibodyAzide and BSA Free [NBP2-94047]

Western Blot: Smad7 AntibodyAzide and BSA Free [NBP2-94047]

Western Blot: Smad7 Antibody [NBP2-94047] - Analysis of extracts of Mouse brain, using Smad7 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP Goat Anti- Rabbit IgG (H+L) at 1:10000dilution. Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk inTBST. Detection: ECL Basic Kit. Exposure time: 30s.
Immunocytochemistry/ Immunofluorescence: Smad7 Antibody - Azide and BSA Free [NBP2-94047]

Immunocytochemistry/ Immunofluorescence: Smad7 Antibody - Azide and BSA Free [NBP2-94047]

Immunocytochemistry/Immunofluorescence: Smad7 Antibody [NBP2-94047] - Analysis of C6 cells using Smad7 at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunohistochemistry-Paraffin: Smad7 Antibody - Azide and BSA Free [NBP2-94047]

Immunohistochemistry-Paraffin: Smad7 Antibody - Azide and BSA Free [NBP2-94047]

Immunohistochemistry-Paraffin: Smad7 Antibody [NBP2-94047] - Paraffin-embedded human placenta using Smad7 .
View 2 more images

Applications for Smad7 Antibody - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50-1:200

Immunohistochemistry

1:50-1:200

Western Blot

1:500-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

Azide and BSA Free

Preservative

0.01% Thimerosal

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Smad7

SMADs are members of the MAD-related family of molecules. MAD-related proteins are a family of intracellular proteins that are essential components in the signaling pathways of the serine/threonine kinase receptors of the transforming growth factor beta superfamily (1). SMADs can be divided into receptor-regulated SMADs (R-SMADs: SMAD1, SMAD2, SMAD5, SMAD8 and SMAD9), common-mediator SMAD (co-SMAD: SMAD4), and inhibitory SMADs (I-SMADs: SMAD6 and SMAD7). SMAD1, SMAD5, SMAD8 and SMAD9 have high degrees of homology and antibodies are available that recognize sequences common to all of them. SMAD8 and SMAD9 are typically used asalternate namesfor one another in the literature. Human SMAD1 is a 465 amino acid protein; GenBank Accession No. AAP36050.1. Human SMAD2 is a 467 amino acid protein; GenBank Accession No. AAC51918.1 Human SMAD3 is a 425 amino acid protein; GenBank Accession No. NP_005893.1 Human SMAD4 is a 552 amino acid protein GenBank Accession No. NP_005350.1. Human SMAD5 is a 465 amino acid protein; GenBank Accession No. AAC50791.1. Human SMAD6 is a 496 amino acid protein; GenBank Accession No. AAH12986.1 Human SMAD7 is a 426 amino acid protein; GenBank Accession No. AAB81354.1 Mouse SMAD8 is a 430 amino acid protein; GenBank Accession No. AAN85445.1 Human SMAD9 is a 430 amino acid protein; GenBank Accession No. NP_005896.1

Long Name

Mothers Against DPP Homolog 7

Alternate Names

MADH7, MADH8

Gene Symbol

SMAD7

Additional Smad7 Products

Product Documents for Smad7 Antibody - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Smad7 Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov

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