Smad9 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-98884
![Western Blot: Smad9 Antibody [NBP2-98884]](https://resources.bio-techne.com/images/products/Smad9-Antibody-Western-Blot-NBP2-98884-img0004.jpg "Western Blot: Smad9 Antibody [NBP2-98884]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Predicted:
Mouse (100%), Rat (100%). Backed by our 100% Guarantee.
Applications
Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for Smad9 Antibody
Immunogen
Produced in rabbits immunized with a synthetic peptide corresponding to the center region of the Human Smad9.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
Scientific Data Images for Smad9 Antibody
Western Blot: Smad9 Antibody [NBP2-98884]
Western Blot: Smad9 Antibody [NBP2-98884] - Anti-Smad9 rabbit polyclonal antibody at 1:500 dilution. Lane A: A549 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 52 kDa. Observed band size: 56 kDa![Immunocytochemistry/ Immunofluorescence: Smad9 Antibody [NBP2-98884]](https://resources.bio-techne.com/images/products/Smad9-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-98884-img0001.jpg "Immunocytochemistry/ Immunofluorescence: Smad9 Antibody [NBP2-98884]")
Immunocytochemistry/ Immunofluorescence: Smad9 Antibody [NBP2-98884]
Immunocytochemistry/Immunofluorescence: Smad9 Antibody [NBP2-98884] - Immunofluorescence staining of Smad9 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-human Smad9 polyclonal antibody (1:5000) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.Applications for Smad9 Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:1500-1:50000
Western Blot
1:500-1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen and protein A Affinity-purified
Formulation
0.2 um filtered solution in PBS
Preservative
No Preservative
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Smad9
Long Name
Mothers Against DPP Homolog 9
Alternate Names
Madh6, MADH9
Gene Symbol
SMAD9
Additional Smad9 Products
Product Documents for Smad9 Antibody
Product Specific Notices for Smad9 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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