SMARCA5/SNF2H Antibody (3F4)

Novus Biologicals, part of Bio-Techne | Catalog # H00008467-M02

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Product Datasheet / COA / SDS

Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 3F4

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all SMARCA5/SNF2H Antibodies »

Product Specifications

Immunogen

SMARCA5 (NP_003592, 59 a.a. ~ 147 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. EEIFDDASPGKQKEIQEPDPTYEEKMQTDRANRFEYLLKQTELFAHFIQPAAQKTPTSPLKMKPGRPRIKKDEKQNLLSVGDYRHRRTE

Specificity

SMARCA5 - SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 5 (3F4)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for SMARCA5/SNF2H Antibody (3F4)

Immunocytochemistry/ Immunofluorescence: SMARCA5/SNF2H Antibody (3F4) [H00008467-M02]

Immunocytochemistry/Immunofluorescence: SMARCA5/SNF2H Antibody (3F4) [H00008467-M02] - Analysis of monoclonal antibody to SMARCA5 on HeLa cell . Antibody concentration 10 ug/ml.

Immunohistochemistry-Paraffin: SMARCA5/SNF2H Antibody (3F4) [H00008467-M02]

Immunohistochemistry-Paraffin: SMARCA5/SNF2H Antibody (3F4) [H00008467-M02] - Analysis of monoclonal antibody to SMARCA5 on formalin-fixed paraffin-embedded human prostate. Antibody concentration 3 ug/ml.

Sandwich ELISA: SMARCA5/SNF2H Antibody (3F4) [H00008467-M02] - Detection limit for recombinant GST tagged SMARCA5 is approximately 3ng/ml as a capture antibody.

Applications for SMARCA5/SNF2H Antibody (3F4)

Application

Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Sandwich ELISA

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Application Notes

Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB. GST tag alone is used as a negative control.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: SMARCA5/SNF2H

The protein encoded by this gene is a member of the SWI/SNF family of proteins. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The protein encoded by this gene is a component of the chromatin remodeling and spacing factor RSF, a facilitator of the transcription of class II genes by RNA polymerase II. The encoded protein is similar in sequence to the Drosophila ISWI chromatin remodeling protein. [provided by RefSeq]

Long Name

SWI/SNF-related, Matrix-associated, Actin-dependent Regulator of Chromatin, Subfamily A, Member 5

Alternate Names

ISWI, SNF2H, WCRF135

Entrez Gene IDs

8467 (Human)

Gene Symbol

SMARCA5

UniProt

NP_003592 (Human)

Additional SMARCA5/SNF2H Products

Product Documents for SMARCA5/SNF2H Antibody (3F4)

Product Datasheets

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COA

SDS

Product Specific Notices for SMARCA5/SNF2H Antibody (3F4)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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