SMARCA5/SNF2H Antibody (6H8I7)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16216

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-16216-100ul
NBP3-16216-20ul
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 6H8I7

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all SMARCA5/SNF2H Antibodies »

Product Summary for SMARCA5/SNF2H Antibody (6H8I7)

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 953-1052 of human SMARCA5/SNF2H (O60264). RFLICMLHKLGFDKENVYDELRQCIRNSPQFRFDWFLKSRTAMELQRRCNTLITLIERENMELEEKEKAEKKKRGPKPSTQKRKMDGAPDGRGRKKKLKL

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SMARCA5/SNF2H Antibody (6H8I7)

Western Blot: SMARCA5/SNF2H Antibody (6H8I7) [NBP3-16216]

Western Blot: SMARCA5/SNF2H Antibody (6H8I7) [NBP3-16216]

Western Blot: SMARCA5/SNF2H Antibody (6H8I7) [NBP3-16216] - Western blot analysis of extracts of Mouse thymus, using SMARCA5/SMARCA5/SNF2H Rabbit mAb (NBP3-16216) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
Immunocytochemistry/ Immunofluorescence: SMARCA5/SNF2H Antibody (6H8I7) [NBP3-16216]

Immunocytochemistry/ Immunofluorescence: SMARCA5/SNF2H Antibody (6H8I7) [NBP3-16216]

Immunocytochemistry/Immunofluorescence: SMARCA5/SNF2H Antibody (6H8I7) [NBP3-16216] - Immunofluorescence analysis of U-2 OS cells using SMARCA5/SMARCA5/SNF2H Rabbit mAb (NBP3-16216) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Western Blot: SMARCA5/SNF2H Antibody (6H8I7) [NBP3-16216]

Western Blot: SMARCA5/SNF2H Antibody (6H8I7) [NBP3-16216]

Western Blot: SMARCA5/SNF2H Antibody (6H8I7) [NBP3-16216] - Western blot analysis of extracts of various cell lines, using SMARCA5/SMARCA5/SNF2H Rabbit mAb (NBP3-16216) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
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Applications for SMARCA5/SNF2H Antibody (6H8I7)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Western Blot

1:500 - 1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: SMARCA5/SNF2H

The SWI/SNF-related, matrix-associated, actin-dependent regulators of chromatin (SMARC), also called BRG1-associated factors (BAFs), have been identified as components of the human SWI/SNF-like chromatin-remodeling protein complexes. SNF2h/ISWI, also known as WCRF135, is the gene product of SMARCA5 that associates with WSTF (Williams Syndrome transcription factor) and several other nuclear proteins to mediate chromatin remodeling. SNF2h is also part of the NoRC (nucleolar remodeling complex).

Long Name

SWI/SNF-related, Matrix-associated, Actin-dependent Regulator of Chromatin, Subfamily A, Member 5

Alternate Names

ISWI, SNF2H, WCRF135

Gene Symbol

SMARCA5

Additional SMARCA5/SNF2H Products

Product Documents for SMARCA5/SNF2H Antibody (6H8I7)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for SMARCA5/SNF2H Antibody (6H8I7)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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