SMARCA5/SNF2H Antibody (JE40-44)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-32963
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # JE40-44
Concentration
1 mg/ml
Product Specifications
Immunogen
Synthetic peptide within Human SMARCA5/SNF2H aa 1,003-1,052 / 1,052. (Uniprot: O60264)
Localization
Nucleus, Chromosome.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
122 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for SMARCA5/SNF2H Antibody (JE40-44)
Western Blot: SMARCA5/SNF2H Antibody (JE40-44) [NBP3-32963]
Western Blot: SMARCA5/SNF2H Antibody (JE40-44) [NBP3-32963] - Western blot analysis of SMARCA5/SNF2H on different lysates with Rabbit anti-SMARCA5/SNF2H antibody (NBP3-32963) at 1/1,000 dilution.Lane 1: HeLa cell lysate
Lane 2: U-2 OS cell lysate
Lane 3: Jurkat cell lysate
Lane 4: HepG2 cell lysate
Lane 5: A549 cell lysate
Lane 6: NIH/3T3 cell lysate
Lane 7: PC-12 cell lysate
Lysates/proteins at 20 ug/Lane.
Predicted band size: 122 kDa
Observed band size: 122 kDa
Exposure time: 1 minute 55 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32963) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.
Applications for SMARCA5/SNF2H Antibody (JE40-44)
Application
Recommended Usage
Western Blot
1:1000
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
1*TBS (pH7.4), 0.05% BSA and 40% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: SMARCA5/SNF2H
Long Name
SWI/SNF-related, Matrix-associated, Actin-dependent Regulator of Chromatin, Subfamily A, Member 5
Alternate Names
ISWI, SNF2H, WCRF135
Gene Symbol
SMARCA5
Additional SMARCA5/SNF2H Products
Product Documents for SMARCA5/SNF2H Antibody (JE40-44)
Product Specific Notices for SMARCA5/SNF2H Antibody (JE40-44)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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