SMARCA5/SNF2H Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-55311

Novus Biologicals, part of Bio-Techne
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NB100-55311
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Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Human

Applications

Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all SMARCA5/SNF2H Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 1002 and the C-terminus (residue 1052) of human SNF2h/ISWI using the numbering given in entry NP_003592.2 (GeneID 8467).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

122 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for SMARCA5/SNF2H Antibody

Immunoprecipitation: SMARCA5/SNF2H Antibody [NB100-55311]

Immunoprecipitation: SMARCA5/SNF2H Antibody [NB100-55311]

Immunoprecipitation: SMARCA5/SNF2H Antibody [NB100-55311] - Detection of Human SNF2h/ISWI by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-SNF2h/ISWI antibody NB100-55311 used for IP at 3 mcg/mg lysate. SNF2h/ISWI was also immunoprecipitated by another rabbit anti-SNF2h/ISWI antibody and NB100-55310, which recognize upstream epitopes. Detection: Chemiluminescence with an exposure time of 10 seconds.

Applications for SMARCA5/SNF2H Antibody

Application
Recommended Usage

Immunoprecipitation

2-5 ug/mg lysate

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: SMARCA5/SNF2H

The SWI/SNF-related, matrix-associated, actin-dependent regulators of chromatin (SMARC), also called BRG1-associated factors (BAFs), have been identified as components of the human SWI/SNF-like chromatin-remodeling protein complexes. SNF2h/ISWI, also known as WCRF135, is the gene product of SMARCA5 that associates with WSTF (Williams Syndrome transcription factor) and several other nuclear proteins to mediate chromatin remodeling. SNF2h is also part of the NoRC (nucleolar remodeling complex).

Long Name

SWI/SNF-related, Matrix-associated, Actin-dependent Regulator of Chromatin, Subfamily A, Member 5

Alternate Names

ISWI, SNF2H, WCRF135

Entrez Gene IDs

8467 (Human)

Gene Symbol

SMARCA5

UniProt

O60264 (Human)

Additional SMARCA5/SNF2H Products

Product Documents for SMARCA5/SNF2H Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for SMARCA5/SNF2H Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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