SMARCA5/SNF2H Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-89692

Novus Biologicals, part of Bio-Techne
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NBP1-89692
NBP1-89692-25ul
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Knockdown Validated, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all SMARCA5/SNF2H Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: SSAAEPPPPPPPESAPSKPAASIASGGSNSSNKGGPEGVAAQAVASAASAGPADAEMEEIFDDASPGKQKEIQEPDPTYEEKMQTDRANRFEYLLKQTELFAHFIQPA

Reactivity Notes

Reactivity reported in scientific literature (PMID: 23240041)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SMARCA5/SNF2H Antibody

Western Blot: SMARCA5/SNF2H Antibody [NBP1-89692]

Western Blot: SMARCA5/SNF2H Antibody [NBP1-89692]

Western Blot: SMARCA5/SNF2H Antibody [NBP1-89692] - Analysis in Rh30 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-SMARCA5 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.
Immunocytochemistry/ Immunofluorescence: SMARCA5/SNF2H Antibody [NBP1-89692]

Immunocytochemistry/ Immunofluorescence: SMARCA5/SNF2H Antibody [NBP1-89692]

Immunocytochemistry/Immunofluorescence: SMARCA5/SNF2H Antibody [NBP1-89692] - Staining of human cell line A-431 shows localization to nucleus & nucleoli fibrillar center. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: SMARCA5/SNF2H Antibody [NBP1-89692]

Immunohistochemistry-Paraffin: SMARCA5/SNF2H Antibody [NBP1-89692]

Immunohistochemistry-Paraffin: SMARCA5/SNF2H Antibody [NBP1-89692] - Staining of human testis shows strong nuclear positivity in cells in seminiferus ducts.
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Applications for SMARCA5/SNF2H Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SMARCA5/SNF2H

The SWI/SNF-related, matrix-associated, actin-dependent regulators of chromatin (SMARC), also called BRG1-associated factors (BAFs), have been identified as components of the human SWI/SNF-like chromatin-remodeling protein complexes. SNF2h/ISWI, also known as WCRF135, is the gene product of SMARCA5 that associates with WSTF (Williams Syndrome transcription factor) and several other nuclear proteins to mediate chromatin remodeling. SNF2h is also part of the NoRC (nucleolar remodeling complex).

Long Name

SWI/SNF-related, Matrix-associated, Actin-dependent Regulator of Chromatin, Subfamily A, Member 5

Alternate Names

ISWI, SNF2H, WCRF135

Gene Symbol

SMARCA5

Additional SMARCA5/SNF2H Products

Product Documents for SMARCA5/SNF2H Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for SMARCA5/SNF2H Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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