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SmarcAL1 Antibody (PCRP-SMARCAL1-1C4)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-20555

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2c Kappa Clone # PCRP-SMARCAL1-1C4

Concentration

0.2 mg/ml

Product Specifications

Immunogen

Recombinant full-length human SmarcAL1 protein

Localization

Nucleus.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2c Kappa

Description

Positive Controls: HeLa whole cell lysate. Human prostate or breast carcinoma.

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. Non-hazardous. No MSDS required.

Scientific Data Images for SmarcAL1 Antibody (PCRP-SMARCAL1-1C4)

SmarcAL1 Antibody (PCRP-SMARCAL1-1C4)

SmarcAL1 Antibody (PCRP-SMARCAL1-1C4)

Analysis of Protein Array containing more than 19,000 full-length human proteins using SMARCAL1 Mouse Monoclonal Antibody (PCRP-SMARCAL1-1C4)
SmarcAL1 Antibody (PCRP-SMARCAL1-1C4)

SmarcAL1 Antibody (PCRP-SMARCAL1-1C4)

Formalin-fixed, paraffin-embedded human tonsil stained with SmarcAL1 antibody (PCRP-SMARCAL1-1C4). Inset: PBS instead of primary antibody; secondary only negative control.
SmarcAL1 Antibody (PCRP-SMARCAL1-1C4)

SmarcAL1 Antibody (PCRP-SMARCAL1-1C4)

Formalin-fixed, paraffin-embedded human kidney carcinoma stained with SmarcAL1 antibody (PCRP-SMARCAL1-1C4). HIER: Tris/EDTA, pH9.0, 45min. Secondary: HRP-polymer, 30min. DAB, 5min.

Applications for SmarcAL1 Antibody (PCRP-SMARCAL1-1C4)

Application
Recommended Usage

Immunohistochemistry-Paraffin

1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes. at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.

Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: SmarcAL1

SmarcAL1 is encoded by this gene is a member of the SWI/SNF family of proteins. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein shows sequence similarity to the E. coli RNA polymerase-binding protein HepA. Mutations in this gene are a cause of Schimke immunoosseous dysplasia (SIOD), an autosomal recessive disorder with the diagnostic features of spondyloepiphyseal dysplasia, renal dysfunction, and T-cell immunodeficiency.

Alternate Names

ATP-driven annealing helicase, EC 3.6.1, EC 3.6.4.-, HARPSWI/SNF-related matrix-associated actin-dependent regulator of chromatinsubfamily A-like protein 1, HepA-related protein, HHARP, SIOD, SMARCA-like protein 1, subfamily a-like 1, Sucrose nonfermenting protein 2-like 1, SWI/SNF related, matrix associated, actin dependent regulator of chromatin

Gene Symbol

SMARCAL1

UniProt

Additional SmarcAL1 Products

Product Documents for SmarcAL1 Antibody (PCRP-SMARCAL1-1C4)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for SmarcAL1 Antibody (PCRP-SMARCAL1-1C4)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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