SmarcAL1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-12599

Novus Biologicals, part of Bio-Techne
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NBP3-12599-100ul
NBP3-12599-50ul
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Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all SmarcAL1 Antibodies »

Product Specifications

Immunogen

Produced in rabbits immunized with E. coli-derived Human SmarcAL1 fragment

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Protein A & Antigen Affinity purified

Scientific Data Images for SmarcAL1 Antibody

Western Blot: SmarcAL1 Antibody [NBP3-12599]

Western Blot: SmarcAL1 Antibody [NBP3-12599]

Western Blot: SmarcAL1 Antibody [NBP3-12599] - Lane A: Jurkat Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:106 kDa(We are unsure as to the identity of these extra bands.)
Immunocytochemistry/ Immunofluorescence: SmarcAL1 Antibody [NBP3-12599]

Immunocytochemistry/ Immunofluorescence: SmarcAL1 Antibody [NBP3-12599]

Immunocytochemistry/Immunofluorescence: SmarcAL1 Antibody [NBP3-12599] - Staining of SMARCAL1 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human SMARCAL1 polyclonal antibody (dilution ratio 1:200) at 4? overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus.
Immunoprecipitation: SmarcAL1 Antibody [NBP3-12599]

Immunoprecipitation: SmarcAL1 Antibody [NBP3-12599]

Immunoprecipitation: SmarcAL1 Antibody [NBP3-12599] - Lane A:0.5 mg Jurkat Whole Cell Lysate4 uL anti-SMARCAL1 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-SMARCAL1 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 106 kDaObserved band size :120 kDa

Applications for SmarcAL1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:500

Immunoprecipitation

1-5ul/mg of lysate

Western Blot

1:1000-1:5000

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

PBS

Preservative

0.03% Proclin 300

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SmarcAL1

SmarcAL1 is encoded by this gene is a member of the SWI/SNF family of proteins. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein shows sequence similarity to the E. coli RNA polymerase-binding protein HepA. Mutations in this gene are a cause of Schimke immunoosseous dysplasia (SIOD), an autosomal recessive disorder with the diagnostic features of spondyloepiphyseal dysplasia, renal dysfunction, and T-cell immunodeficiency.

Alternate Names

ATP-driven annealing helicase, EC 3.6.1, EC 3.6.4.-, HARPSWI/SNF-related matrix-associated actin-dependent regulator of chromatinsubfamily A-like protein 1, HepA-related protein, HHARP, SIOD, SMARCA-like protein 1, subfamily a-like 1, Sucrose nonfermenting protein 2-like 1, SWI/SNF related, matrix associated, actin dependent regulator of chromatin

Gene Symbol

SMARCAL1

Additional SmarcAL1 Products

Product Documents for SmarcAL1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for SmarcAL1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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