SMARCC1 Antibody (1R7I6)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16367

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-16367-100ul
NBP3-16367-20ul
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Applications

Chromatin Immunoprecipitation (ChIP), Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 1R7I6

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all SMARCC1 Antibodies »

Product Summary for SMARCC1 Antibody (1R7I6)

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 700-800 of human SMARCC1 (Q92922). AFLASVVDPRVASAAAKAALEEFSRVREEVPLELVEAHVKKVQEAARASGKVDPTYGLESSCIAGTGPDEPEKLEGAEEEKMEADPDGQQPEKAENKVENE

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SMARCC1 Antibody (1R7I6)

Western Blot: SMARCC1 Antibody (1R7I6) [NBP3-16367]

Western Blot: SMARCC1 Antibody (1R7I6) [NBP3-16367]

Western Blot: SMARCC1 Antibody (1R7I6) [NBP3-16367] - Western blot analysis of extracts of various cell lines, using SMARCC1 Rabbit mAb (NBP3-16367) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.
Immunocytochemistry/ Immunofluorescence: SMARCC1 Antibody (1R7I6) [NBP3-16367]

Immunocytochemistry/ Immunofluorescence: SMARCC1 Antibody (1R7I6) [NBP3-16367]

Immunocytochemistry/Immunofluorescence: SMARCC1 Antibody (1R7I6) [NBP3-16367] - Immunofluorescence analysis of NIH-3T3 cells using SMARCC1 Rabbit mAb (NBP3-16367) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Chromatin Immunoprecipitation: SMARCC1 Antibody (1R7I6) [NBP3-16367] - Chromatin immunoprecipitation analysis of extracts of MCF7 beta-estradiol cells, using SMARCC1 antibody (NBP3-16367) and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
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Applications for SMARCC1 Antibody (1R7I6)

Application
Recommended Usage

Chromatin Immunoprecipitation (ChIP)

1:100 - 1:500

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Immunoprecipitation

1:100 - 1:500

Western Blot

1:500 - 1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: SMARCC1

The SWI/SNF-related, matrix-associated, actin-dependent regulators of chromatin (SMARC), also called BRG1-associated factors (BAFs), have been identified as components of the human SWI/SNF-like chromatin-remodeling protein complexes. SMARCC1/BAF155 is part of the BAF complex that associates with SMARCA2/Brm and SMARCA4/Brg. The mouse homolog is Srg3 and has been found to be required for embryogenesis and brain development. SMARCC1 is also identified as SWI/SNF complex 155 kDa subunit, and BRG1-associated factor 155.

Alternate Names

BAF155SWI/SNF-related matrix-associated actin-dependent regulator of chromatinsubfamily C member 1, BRG1-associated factor 155, chromatin remodeling complex BAF155 subunit, CRACC1, mammalian chromatin remodeling complex BRG1-associated factor 155, Rsc8, SRG3, subfamily c, member 1, SWI/SNF complex 155 kDa subunit, SWI/SNF complex subunit SMARCC1, SWI/SNF related, matrix associated, actin dependent regulator of chromatin, SWI3

Gene Symbol

SMARCC1

Additional SMARCC1 Products

Product Documents for SMARCC1 Antibody (1R7I6)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for SMARCC1 Antibody (1R7I6)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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