SMARCC1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-20415

Novus Biologicals, part of Bio-Techne
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NBP2-20415
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Key Product Details

Validated by

Knockout/Knockdown, Biological Validation

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Mouse

Applications

Validated:

Chromatin Immunoprecipitation (ChIP), Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all SMARCC1 Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human SMARCC1. The exact sequence is proprietary.

Localization

Nucleus

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

123 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for SMARCC1 Antibody

Western Blot: SMARCC1 Antibody [NBP2-20415]

Western Blot: SMARCC1 Antibody [NBP2-20415]

Western Blot: SMARCC1 Antibody [NBP2-20415] - Wild-type (WT) and SMARCC1 knockout (KO) HeLa cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with SMARCC1 antibody diluted at 1:5000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Immunohistochemistry-Paraffin: SMARCC1 Antibody [NBP2-20415]

Immunohistochemistry-Paraffin: SMARCC1 Antibody [NBP2-20415]

Immunohistochemistry-Paraffin: SMARCC1 Antibody [NBP2-20415] - Paraffin-embedded rat hind brain dilution: 1:500.
Chromatin Immunoprecipitation: SMARCC1 Antibody [NBP2-20415] - Cross-linked ChIP was performed with MF-7 chromatin extract treated with B-estradiol (10 nM for 45 min) and 5 ug of either control rabbit IgG or anti-SMARCC1 antibody. The precipitated DNA was detected by PCR with primer set targeting to ESR1 or Sat2.
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Applications for SMARCC1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Immunoprecipitation

1:100-1:500

Western Blot

1:500-1:20000
Application Notes
ChIP assay Assay-dependent dilution

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS (pH 7), 20% Glycerol

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: SMARCC1

The protein encoded by this gene is a member of the SWI/SNF family of proteins, whose members display helicase and ATPase activities and which are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein is part of the large ATP-dependent chromatin remodeling complex SNF/SWI and contains a predicted leucine zipper motif typical of many transcription factors. [provided by RefSeq]

Alternate Names

BAF155SWI/SNF-related matrix-associated actin-dependent regulator of chromatinsubfamily C member 1, BRG1-associated factor 155, chromatin remodeling complex BAF155 subunit, CRACC1, mammalian chromatin remodeling complex BRG1-associated factor 155, Rsc8, SRG3, subfamily c, member 1, SWI/SNF complex 155 kDa subunit, SWI/SNF complex subunit SMARCC1, SWI/SNF related, matrix associated, actin dependent regulator of chromatin, SWI3

Gene Symbol

SMARCC1

UniProt

Q92922 (Human)

Additional SMARCC1 Products

Product Documents for SMARCC1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for SMARCC1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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