SMARCC1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-98777

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all SMARCC1 Antibodies »

Product Summary for SMARCC1 Antibody

Immunogen

Produced in rabbits immunized with a synthetic peptide corresponding to the center region of the Human SMARCC1.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for SMARCC1 Antibody

Western Blot: SMARCC1 Antibody [NBP2-98777]

Western Blot: SMARCC1 Antibody [NBP2-98777]

Western Blot: SMARCC1 Antibody [NBP2-98777] - Anti-SMARCC1 rabbit polyclonal antibody at 1:500 dilution. Lane A: HeLa Whole Cell Lysate Lane B: U-87MG Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 123 kDa. Observed band size: 150 kDa
Immunocytochemistry/ Immunofluorescence: SMARCC1 Antibody [NBP2-98777]

Immunocytochemistry/ Immunofluorescence: SMARCC1 Antibody [NBP2-98777]

Immunocytochemistry/Immunofluorescence: SMARCC1 Antibody [NBP2-98777] - Immunofluorescence staining of SMARCC1 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-human SMARCC1 polyclonal antibody (dilution ratio 1:1000) at 4C overnight. Then cells were stained with the Alexa Fluor$488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to nucleus.
Immunohistochemistry-Paraffin: SMARCC1 Antibody [NBP2-98777]

Immunohistochemistry-Paraffin: SMARCC1 Antibody [NBP2-98777]

Immunohistochemistry-Paraffin: SMARCC1 Antibody [NBP2-98777] - Immunochemical staining of human SMARCC1 in human kidney with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.

Applications for SMARCC1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:300-1:10000

Immunohistochemistry-Paraffin

1:500-1:2000

Western Blot

1:500-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

0.2 um filtered solution in PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SMARCC1

The SWI/SNF-related, matrix-associated, actin-dependent regulators of chromatin (SMARC), also called BRG1-associated factors (BAFs), have been identified as components of the human SWI/SNF-like chromatin-remodeling protein complexes. SMARCC1/BAF155 is part of the BAF complex that associates with SMARCA2/Brm and SMARCA4/Brg. The mouse homolog is Srg3 and has been found to be required for embryogenesis and brain development. SMARCC1 is also identified as SWI/SNF complex 155 kDa subunit, and BRG1-associated factor 155.

Alternate Names

BAF155SWI/SNF-related matrix-associated actin-dependent regulator of chromatinsubfamily C member 1, BRG1-associated factor 155, chromatin remodeling complex BAF155 subunit, CRACC1, mammalian chromatin remodeling complex BRG1-associated factor 155, Rsc8, SRG3, subfamily c, member 1, SWI/SNF complex 155 kDa subunit, SWI/SNF complex subunit SMARCC1, SWI/SNF related, matrix associated, actin dependent regulator of chromatin, SWI3

Gene Symbol

SMARCC1

Additional SMARCC1 Products

Product Documents for SMARCC1 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for SMARCC1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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