SMARCC2/BAF170 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-90017

Novus Biologicals, part of Bio-Techne
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NBP1-90017
NBP1-90017-25ul
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Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Validated:

Human

Predicted:

Mouse (90%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all SMARCC2/BAF170 Antibodies »

Product Summary for SMARCC2/BAF170 Antibody

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: AFGLESSGIAGTTSDEPERIEESGNDEARVEGQATDEKKEPKEPREGGGAIEEEAKEKTSEAPKKDEEKGKEGDSEKESEKSDGDPIVDPEKEKEPKEGQEEVLKEVVESEGERKTKVERDIGEGNLSTAA

Reactivity Notes

Rat (88%).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SMARCC2/BAF170 Antibody

Western Blot: SMARCC2/BAF170 Antibody [NBP1-90017]

Western Blot: SMARCC2/BAF170 Antibody [NBP1-90017]

Western Blot: SMARCC2/BAF170 Antibody [NBP1-90017] - Analysis using Anti-SMARCC2/BAF170 antibody NBP1-90017 (A) shows similar pattern to a competitors Anti-SMARCC2/BAF170 antibody (B)
Immunocytochemistry/ Immunofluorescence: SMARCC2/BAF170 Antibody [NBP1-90017]

Immunocytochemistry/ Immunofluorescence: SMARCC2/BAF170 Antibody [NBP1-90017]

Immunocytochemistry/Immunofluorescence: SMARCC2/BAF170 Antibody [NBP1-90017] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: SMARCC2/BAF170 Antibody [NBP1-90017]

Immunohistochemistry-Paraffin: SMARCC2/BAF170 Antibody [NBP1-90017]

Immunohistochemistry-Paraffin: SMARCC2/BAF170 Antibody [NBP1-90017] - Staining of human stomach shows strong nuclear positivity in glandular cells.

Applications for SMARCC2/BAF170 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200-1:500

Western Blot

0.04 - 0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SMARCC2/BAF170

SMARCC2 / BAF170 is encoded by this gene is a member of the SWI/SNF family of proteins, whose members display helicase and ATPase activities and which are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein is part of the large ATP-dependent chromatin remodeling complex SNF/SWI and contains a predicted leucine zipper motif typical of many transcription factors. Two transcript variants encoding different isoforms have been found for this gene.

Alternate Names

BAF170DKFZp313D0632, BRG1-associated factor 170, chromatin remodeling complex BAF170 subunit, CRACC2, mammalian chromatin remodeling complex BRG1-associated factor 170, Rsc8, subfamily c, member 2, SWI/SNF complex 170 kDa subunit, SWI/SNF complex subunit SMARCC2, SWI/SNF related, matrix associated, actin dependent regulator of chromatin, SWI/SNF-related matrix-associated actin-dependent regulator of chromatinsubfamily C member 2, SWI3-like protein

Gene Symbol

SMARCC2

Additional SMARCC2/BAF170 Products

Product Documents for SMARCC2/BAF170 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for SMARCC2/BAF170 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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