SMARCD3 Antibody (5F12)

Novus Biologicals, part of Bio-Techne | Catalog # H00006604-M02

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human, Mouse

Applications

ELISA, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 5F12

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all SMARCD3 Antibodies »

Product Specifications

Immunogen

SMARCD3 (NP_001003801, 385 a.a. ~ 483 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. ISALDSKIHETIESINQLKIQRDFMLSFSRDPKGYVQDLLRSQSRDLKVMTDVAGNPEEERRAEFYHQPWSQEAVSRYFYCKIQQRRQELEQSLVVRNT

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Specificity

SMARCD3 - SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily d, member 3 (5F12)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for SMARCD3 Antibody (5F12)

Western Blot: SMARCD3 Antibody (5F12) [H00006604-M02]

Western Blot: SMARCD3 Antibody (5F12) [H00006604-M02]

Western Blot: SMARCD3 Antibody (5F12) [H00006604-M02] - SMARCD3 monoclonal antibody (M02), clone 5F12. Analysis of SMARCD3 expression in NIH/3T3.
Western Blot: SMARCD3 Antibody (5F12) [H00006604-M02]

Western Blot: SMARCD3 Antibody (5F12) [H00006604-M02]

Western Blot: SMARCD3 Antibody (5F12) [H00006604-M02] - SMARCD3 monoclonal antibody (M02), clone 5F12 Analysis of SMARCD3 expression in K-562.
Sandwich ELISA: SMARCD3 Antibody (5F12) [H00006604-M02] - Detection limit for recombinant GST tagged SMARCD3 is approximately 0.3ng/ml as a capture antibody.

Applications for SMARCD3 Antibody (5F12)

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Sandwich ELISA

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: SMARCD3

The protein encoded by this gene is a member of the SWI/SNF family of proteins, whose members display helicase and ATPase activities and which are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein is part of the large ATP-dependent chromatin remodeling complex SNF/SWI and has sequence similarity to the yeast Swp73 protein. Multiple alternatively spliced transcript variants have been found for this gene, but the biological validity of some variants has not been determined.

Alternate Names

60 kDa BRG-1/Brm-associated factor subunit C, 60kDa BRG-1/Brm associated factor subunit c, BAF60Cchromatin remodeling complex BAF60C subunit, BRG1-associated factor 60C, CRACD3, mammalian chromatin remodeling complex BRG1-associated factor 60C, MGC111010, Rsc6p, subfamily d, member 3, SWI/SNF complex 60 kDa subunit C, SWI/SNF related, matrix associated, actin dependent regulator of chromatin, SWI/SNF-related matrix-associated actin-dependent regulator of chromatinsubfamily D member 3, Swp73-like protein

Entrez Gene IDs

6604 (Human)

Gene Symbol

SMARCD3

UniProt

NP_001003801 (Human)

Additional SMARCD3 Products

Product Documents for SMARCD3 Antibody (5F12)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for SMARCD3 Antibody (5F12)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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