Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Knockdown Validated, Western Blot

Cited:

Block/Neutralize, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Knockdown Validated, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all SMC2 Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 1150 and the C-terminus (residue 1197) of human Structural Maintenance of Chromosomes 2 using the numbering given in entry NP_006435.1 (GeneID 10592).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SMC2 Antibody

Western Blot: SMC2 Antibody [NB100-373]

Western Blot: SMC2 Antibody [NB100-373]

Western Blot: SMC2 Antibody [NB100-373] - Detection of Human SMC2 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SMC2 antibody NB100-373 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.
Immunocytochemistry/ Immunofluorescence: SMC2 Antibody [NB100-373]

Immunocytochemistry/ Immunofluorescence: SMC2 Antibody [NB100-373]

Immunocytochemistry/Immunofluorescence: SMC2 Antibody [NB100-373] - SMC2 in HaCaT keratinocytes treated with control shRNA (upper panels) and treated with Nesprin-2-SMC domain specific shRNA (lower panels). Nesprin-2 was detected with mAb K20-478. Bar, 10 um. Image collected and cropped by CiteAb from the following publication (https://www.hindawi.com/journals/ijcb/2017/8607532/), licensed under a CC-BY license.
Western Blot: SMC2 Antibody [NB100-373]

Western Blot: SMC2 Antibody [NB100-373]

Western Blot: SMC2 Antibody [NB100-373] - Detection of Human SMC2 on HeLa whole cell lysate using NB100-373.
View 1 more images

Applications for SMC2 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:200-1:400

Immunoprecipitation

2-10 ug/mg lysate

Western Blot

1:2000 - 1:10000
Application Notes
NB 100-373 may be used for Western Blot, where a band at ~155 kDa is seen, representing SMC2 protein. *The investigator should determine the optimal working dilution for a specific application.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: SMC2

Structural Maintenance of Chromosome (SMC) proteins are required for nuclear processes, including chromosome condensation, chromatid cohesion, and DNA repair. Eukaryotic proteins are capable of binding DNA and possess a DNA-stimulated ATPase activity.

Alternate Names

CAP-E, CAPESMC2 structural maintenance of chromosomes 2-like 1 (yeast), chromosome-associated protein E, FLJ10093, hCAP-EXCAP-E homolog, SMC protein 2, SMC-2, SMC2 (structural maintenance of chromosomes 2, yeast)-like 1, SMC2 structural maintenance of chromosomes 2-like 1, SMC2L1, structural maintenance of chromosomes (SMC) family member, structural maintenance of chromosomes 2, structural maintenance of chromosomes protein 2

Entrez Gene IDs

10592 (Human)

Gene Symbol

SMC2

UniProt

NP_006435.1 (Human)

Additional SMC2 Products

Product Documents for SMC2 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for SMC2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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