Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all SMC3 Antibodies »

Product Summary for SMC3 [p Ser383] Antibody

Immunogen

Immunogen for this antibody was a phosphorylated synthetic peptide, which represented a portion of human Structural Maintenance of Chromosomes 3 (GeneID 9126) around serine 383 according to the numbering given in entry NP_005436.1.

Modification

p Ser383

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SMC3 [p Ser383] Antibody

Immunoprecipitation: SMC3 [p Ser383] Antibody [NB100-208]

Immunoprecipitation: SMC3 [p Ser383] Antibody [NB100-208]

Immunoprecipitation: SMC3 [p Ser383] Antibody [NB100-208] - Immunoprecipitation of Human Phospho SMC3 (Ser383). Samples: Nuclear extracts (50 ug) from HeLa cells that were irradiated with 10 Gy of ionizing radiation or mock irradiated. Antibodies: Rabbit anti-SMC3 pSer383 NB100-208 was used at 4 ug/mg lysate to IP phospho SMC3 (Ser383). Rabbit anti-SMC3 NB100-208 was used at 4 ug/mg lysate for IP and at 0.5 ug/ml for WB. Detection: Chemiiluminescence.

Applications for SMC3 [p Ser383] Antibody

Application
Recommended Usage

Immunoprecipitation

2 - 6 ug/mg of lysate
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: SMC3

Involved in chromosome cohesion during cell cycle and in DNA repair. Central component of cohesin complex. The cohesin complex is required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. The cohesin complex may also play a role in spindle pole assembly during mitosis and in chromosome movement.

Alternate Names

Bamacan, BAMSMC protein 3, Basement membrane-associated chondroitin proteoglycan, BMH, Chondroitin sulfate proteoglycan 6, chondroitin sulfate proteoglycan 6 (bamacan), Chromosome-associated polypeptide, CSPG6structural maintenance of chromosomes protein 3, hCAP, HCAPbamacan, SMC-3, SMC3L1CDLS3, structural maintenance of chromosomes 3

Entrez Gene IDs

9126 (Human)

Gene Symbol

SMC3

UniProt

NP_005436.1. (Human)

Additional SMC3 Products

Product Documents for SMC3 [p Ser383] Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for SMC3 [p Ser383] Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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