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SMCHD1 Antibody (CL4270)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-59046

Novus Biologicals, part of Bio-Techne
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NBP2-59046
NBP2-59046-25ul

Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (97%), Rat (98%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # CL4270

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to the following amino acid sequence:DNGRGMTSKQLNNWAVYRLSKFTRQGDFESDHSGYVRPVPVPRSLNSDISYFGVGGKQAVFFVGQSARMISKPADSQDVHELVLSKED

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for SMCHD1 Antibody (CL4270)

Immunocytochemistry/ Immunofluorescence: SMCHD1 Antibody (CL4270) [NBP2-59046]

Immunocytochemistry/ Immunofluorescence: SMCHD1 Antibody (CL4270) [NBP2-59046]

Immunocytochemistry/Immunofluorescence: SMCHD1 Antibody (CL4270) [NBP2-59046] - Staining of U-2 OS cells using the Anti-SMCHD1 monoclonal antibody, showing specific staining in the nucleoplasm in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).
Immunohistochemistry-Paraffin: SMCHD1 Antibody (CL4270) [NBP2-59046]

Immunohistochemistry-Paraffin: SMCHD1 Antibody (CL4270) [NBP2-59046]

Immunohistochemistry-Paraffin: SMCHD1 Antibody (CL4270) [NBP2-59046] - Staining of human skeletal muscle shows no positivity as expected (negative control).
Immunohistochemistry-Paraffin: SMCHD1 Antibody (CL4270) [NBP2-59046]

Immunohistochemistry-Paraffin: SMCHD1 Antibody (CL4270) [NBP2-59046]

Immunohistochemistry-Paraffin: SMCHD1 Antibody (CL4270) [NBP2-59046] - Staining of human testis shows moderate to strong nuclear positivity in seminiferous tubules cells.

Applications for SMCHD1 Antibody (CL4270)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SMCHD1

Alternate Names

DKFZp686O0631, KIAA0650, structural maintenance of chromosomes flexible hinge domain containing 1, structural maintenance of chromosomes flexible hinge domain-containing protein 1

Gene Symbol

SMCHD1

Additional SMCHD1 Products

Product Documents for SMCHD1 Antibody (CL4270)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for SMCHD1 Antibody (CL4270)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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