SMCHD1 Antibody

Western Blot: SMCHD1 Antibody [NBP1-49968]

Western Blot: SMCHD1 Antibody [NBP1-49968] - Detection of Human SmcHD1 by Western Blot and Immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 mcg) cells. Antibodies: Affinity purified rabbit anti-SmcHD1 antibody used for WB at 0.04 mcg/ml (A) and 0.4 mcg/ml (B) and used for IP at 3 mcg/mg lysate. SmcHD1 was also immunoprecipitated by rabbit anti-SmcHD1 antibody which recognizes a downstream epitope. For blotting immunoprecipitated SmcHD1 was used. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 10 seconds (B).

Immunohistochemistry-Paraffin: SMCHD1 Antibody [NBP1-49968]

Immunohistochemistry-Paraffin: SMCHD1 Antibody [NBP1-49968] - Sample: FFPE section of human testicular seminoma (left) and non-small cell lung cancer (right). Antibody: Affinity purified rabbit anti- SmcHD1 used at a dilution of 1:200 (1ug/ml). Detection: Vector Laboratories ImmPACT NovaRED Peroxidase Substrate