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SMCX Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-55327

Novus Biologicals, part of Bio-Techne
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NB100-55327

Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Human

Applications

Chromatin Immunoprecipitation Sequencing, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

0.2 mg/ml

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 275 and 325 of human Jumonji, AT rich interactive domain 1C using the numbering given in entry NP_004178.2 (GeneID 8242).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SMCX Antibody

Western Blot: SMCX Antibody [NB100-55327]

Western Blot: SMCX Antibody [NB100-55327]

Western Blot: SMCX Antibody [NB100-55327] - Detection of Human and Mouse Jarid1C by Western Blot. Samples: Whole cell lysate (50, 15 ug) from HeLa and (50 ug) from 293T, Jurkat, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Jarid1C antibody NB100-55327 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Chromatin Immunoprecipitation Sequencing: SMCX Antibody [NB100-55327] - Localization of Jarid1C Binding Sites by ChIP-sequencing. Chromatin from K562 cells was immunoprecipitated with anti-Jarid1C antibody NB100-55327 and analyzed by DNA sequencing. The figure illustrates the peak distribution of Jarid1C binding within a 500 Kb region of chromosome 1 as detected using anti-Jarid1C antibody NB100-55327. ChIP-seq validation performed by Diogenode, Denville, NJ.
Immunoprecipitation: SMCX Antibody [NB100-55327]

Immunoprecipitation: SMCX Antibody [NB100-55327]

Immunoprecipitation: SMCX Antibody [NB100-55327] - Detection of human Jarid1C (SMCX) by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Jarid1C antibody NB100-55327 used for IP at 6 ug per reaction. Jarid1C was also immunoprecipitated by rabbit anti-Jarid1C antibody NB100-55328. For blotting immunoprecipitated Jarid1C, NB100-55327 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Applications for SMCX Antibody

Application
Recommended Usage

Immunoprecipitation

1:10-1:500

Western Blot

1:2000-1:10000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS and 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: SMCX

This gene is a member of the SMCY homolog family and encodes a protein with one ARID domain, one JmjC domain, one JmjN domain and two PHD-type zinc fingers. The DNA-binding motifs suggest this protein is involved in the regulation of transcription and chromatin remodeling. Mutations in this gene have been associated with X-linked mental retardation. Alternatively spliced variants that encode different protein isoforms have been described but the full-length nature of only one has been determined.

Long Name

Lysine-specific demethylase 5C

Alternate Names

DXS1272E, JARID1C, KDM5C, Protein SmcX, Protein Xe169, XE169

Entrez Gene IDs

8242 (Human)

Gene Symbol

KDM5C

Additional SMCX Products

Product Documents for SMCX Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for SMCX Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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