Smek1 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NB100-2879
![Western Blot: Smek1 Antibody [NB100-2879]](https://resources.bio-techne.com/images/products/Smek1-Antibody-Western-Blot-NB100-2879-img0005.jpg "Western Blot: Smek1 Antibody [NB100-2879]")
Key Product Details
Validated by
Independent Antibodies, Biological Validation
Species Reactivity
Human
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
0.2 mg/ml
Product Specifications
Immunogen
The immunogen recognized by this antibody maps to a region between residue 770 and the C-terminus (residue 820) of human Protein Phosphatase 4, Regulatory Subunit 3 Alpha (KIAA2010) using the numbering given in entry NP_115949.3
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Smek1 Antibody
![Immunohistochemistry: Smek1 Antibody [NB100-2879]](https://resources.bio-techne.com/images/products/Smek1-Antibody-Immunohistochemistry-NB100-2879-img0007.jpg "Immunohistochemistry: Smek1 Antibody [NB100-2879]")
Immunohistochemistry: Smek1 Antibody [NB100-2879]
Immunohistochemistry: Smek1 Antibody [NB100-2879] - Sample: FFPE sections of human colon carcinoma (left) and breast carcinoma (right). Antibody: Affinity purified rabbit anti- PPP4R3 Alpha used at a dilution of 1:200 (1ug/ml) and 1:80 (2.5 ug/ml). Detection: DAB and Red-fluorescent Goat anti-Rabbit IgG-heApplications for Smek1 Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:50-1:250
Immunohistochemistry
1:10-1:500
Immunohistochemistry-Paraffin
1:10-1:500
Immunoprecipitation
2-5 ug/mg lysate
Western Blot
1:2000-1:10000
Application Notes
Epitope retrieval with Tris-EDTA pH 9.0 is recommended for FFPE tissue sections.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: Smek1
Alternate Names
FLFL1, FLJ20707, KIAA2010MSTP033, PP4R3A, PPP4R3A, serine/threonine-protein phosphatase 4 regulatory subunit 3A, SMEK homolog 1, SMEK homolog 1, suppressor of mek1 (Dictyostelium), smk1, smk-1
Gene Symbol
PPP4R3A
UniProt
Additional Smek1 Products
Product Documents for Smek1 Antibody
Product Specific Notices for Smek1 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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