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Smek1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-68163

Novus Biologicals, part of Bio-Techne
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NB100-68163

Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Canine (100%), Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

Product Specifications

Immunogen

Peptide with sequence C-EQQRERQDNPKLD corresponding to internal region according to NP_115949.3.

Epitope

EQQRERQDNPKLD

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Smek1 Antibody

Western Blot: Smek1 Antibody [NB100-68163]

Western Blot: Smek1 Antibody [NB100-68163]

Western Blot: Smek1 Antibody [NB100-68163] - (0.3ug/ml) staining of Human Pancreas lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Applications for Smek1 Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:16000

Western Blot

0.3 - 1 ug/ml
Application Notes
WB: Approx. 110 kDa band observed in human pancreas lysates (calculated MW of 93.9 kDa band according to NP_115949.3). In transfected HEK293 transiently expressing SMEK1 a band of approx. 95 kDa is observed. This band is not observed in the non-transfected HEK293.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Smek1

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Alternate Names

FLFL1, FLJ20707, KIAA2010MSTP033, PP4R3A, PPP4R3A, serine/threonine-protein phosphatase 4 regulatory subunit 3A, SMEK homolog 1, SMEK homolog 1, suppressor of mek1 (Dictyostelium), smk1, smk-1

Entrez Gene IDs

55671 (Human); 68734 (Mouse); 314388 (Rat)

Gene Symbol

PPP4R3A

Additional Smek1 Products

Product Documents for Smek1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Smek1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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