SMG9 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-13354

Novus Biologicals, part of Bio-Techne
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NBP2-13354
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (99%), Rat (96%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all SMG9 Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to the amino acids: SLYRFLQTAEMVKPSTPSPSHESSSSSGSDEGTEYYPHLVFLQNKARREDFCPRKLRQMHLMIDQLMAHSHLRYKGTLSMLQCNV

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SMG9 Antibody

Western Blot: SMG9 Antibody [NBP2-13354]

Western Blot: SMG9 Antibody [NBP2-13354]

Western Blot: SMG9 Antibody [NBP2-13354] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4
Immunocytochemistry/ Immunofluorescence: SMG9 Antibody [NBP2-13354]

Immunocytochemistry/ Immunofluorescence: SMG9 Antibody [NBP2-13354]

Immunocytochemistry/Immunofluorescence: SMG9 Antibody [NBP2-13354] - Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol & mitochondria.
Immunohistochemistry-Paraffin: SMG9 Antibody [NBP2-13354]

Immunohistochemistry-Paraffin: SMG9 Antibody [NBP2-13354]

Immunohistochemistry-Paraffin: SMG9 Antibody [NBP2-13354] - Staining of human stomach shows moderate cytoplasmic positivity in glandular cells.

Applications for SMG9 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SMG9

SMG9 is a subunit of the SMG-1 complex. SMG-1 and Upf1 are part of a surveillance complex that participates in nonsense-mediated mRNA decay (NMD) to degrade mRNAs containing premature translation initiation codons (PTCs).

Alternate Names

chromosome 19 open reading frame 61, DKFZp564H1322, F17127_1, FLJ12886, protein SMG9, Protein smg-9 homolog, SMG9

Gene Symbol

SMG9

Additional SMG9 Products

Product Documents for SMG9 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for SMG9 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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