SMOC-1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-15123

Novus Biologicals, part of Bio-Techne
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NBP2-15123
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Bovine (95%), Mouse (96%), Rat (95%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all SMOC-1 Antibodies »

Product Summary for SMOC-1 Antibody

Immunogen

Recombinant protein encompassing a sequence within the center region of human SMOC-1. The exact sequence is proprietary.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Xenopus laevis (81%).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

48 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for SMOC-1 Antibody

Western Blot: SMOC-1 Antibody [NBP2-15123]

Western Blot: SMOC-1 Antibody [NBP2-15123]

Western Blot: SMOC-1 Antibody [NBP2-15123] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with SMOC1 antibody [N2C3] diluted at 1:500.
Immunohistochemistry-Paraffin: SMOC-1 Antibody [NBP2-15123]

Immunohistochemistry-Paraffin: SMOC-1 Antibody [NBP2-15123]

Immunohistochemistry-Paraffin: SMOC-1 Antibody [NBP2-15123] - Human colon carcinoma, using SMOC1 antibody at 1:250 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Applications for SMOC-1 Antibody

Application
Recommended Usage

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS (pH 7), 20% Glycerol, 1% BSA

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: SMOC-1

SMOC-1 (secreted, or SPARC-related, modular calcium-binding protein 1) is a 70-90 kDa secreted glycoprotein that is a member of the SPARC family of matricellular molecules. Mature mouse SMOC-1 is 427 amino acids (aa) in length. It contains one Kazal-like domain (aa 42-86), two thyroglobulin type-1 segments (aa 91-157 and 223-291) and two functional EF-hand sequences (aa 358-393 and 395-430). Two splice variants contain an insertion of eleven aa after Lys174, with one of these also including an alternate start site at Met65. Mature mouse SMOC-1 shares 99% aa identity with rat SMOC-1 and 92% aa identity with human, canine and bovine SMOC-1. The principal difference between rodents and other mammals is an additional 19 aa near the C-terminus of rodent SMOC-1. Like other matricellular proteins, SMOC-1 is primarily expressed in basement membranes, although it has also been found in other extracellular matrices and the oocyte zona pellucida. It is present early in mouse embryogenesis, and is produced by cells deriving from all three germ layers. Recombinant bacterially produced human SMOC-1 and SMOC-2 were both shown to bind the acute phase protein, C-reactive protein, and the adhesion proteins, fibulin and vitronectin. A signaling role for SMOC-1 was shown in rat mesangial cells: induction of nitric oxide in response to inflammatory cytokines downregulates SMOC-1 which, in turn, downregulates expression of TGF-beta and TGF-beta-regulated genes. This mechanism is proposed to limit the profibrotic effects of TGF-beta, for example in the glomerulus. In Xenopus, the SMOC paralog has been shown to antagonize BMP activity.

Long Name

Secreted Modular Calcium-binding Protein 1

Alternate Names

SMOC1, SPARC-related modular calcium-binding protein 1

Gene Symbol

SMOC1

UniProt

Q9H4F8 (Human)

Additional SMOC-1 Products

Product Documents for SMOC-1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for SMOC-1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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