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SMPD3/NSMase2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-69344

Novus Biologicals, part of Bio-Techne
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NBP1-69344

Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

0.5 mg/ml

Product Specifications

Immunogen

Synthetic peptides corresponding to SMPD3(sphingomyelin phosphodiesterase 3, neutral membrane (neutral sphingomyelinase II)) The peptide sequence was selected from the middle region of SMPD3 (NP_061137). Peptide sequence RPPEADDPVPGGQARNGAGGGPRGQTPNHNQQDGDSGSLGSPSASRESLV The peptide sequence for this immunogen was taken from within the described region.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

71 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

The addition of 50% glycerol is optional for those storing this antibody at -20C and not aliquoting smaller units. However, please note that glycerol may interrupt some downstream antibody applications and should be added with caution.

Scientific Data Images for SMPD3/NSMase2 Antibody

Western Blot: SMPD3/NSMase2 Antibody [NBP1-69344]

Western Blot: SMPD3/NSMase2 Antibody [NBP1-69344]

Western Blot: NSMASE2 Antibody [NBP1-69344] - This Anti-SMPD3 antibody was used in Western Blot of Fetal Lung tissue lysate at a concentration of 1ug/ml.

Applications for SMPD3/NSMase2 Antibody

Application
Recommended Usage

Western Blot

1.0 ug/ml

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 2% Sucrose

Preservative

0.09% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SMPD3

SMPD3 catalyzes the hydrolysis of sphingomyelin to form ceramide and phosphocholine. Ceramide mediates numerous cellular functions, such as apoptosis and growth arrest, and is capable of regulating these 2 cellular events independently. Also hydrolyzes sphingosylphosphocholine. SMPD3 regulates the cell cycle by acting as a growth suppressor in confluent cells. It probably acts as a regulator of postnatal development and participates in bone and dentin mineralization.

Long Name

Sphingomyelin Phosphodiesterase 3

Alternate Names

NSMASE2

Entrez Gene IDs

55512 (Human)

Gene Symbol

SMPD3

UniProt

Additional SMPD3 Products

Product Documents for SMPD3/NSMase2 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for SMPD3/NSMase2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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