SMPD4 Antibody - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-93253
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Cited:
Human
Applications
Validated:
ELISA, Immunocytochemistry/ Immunofluorescence, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
Azide and BSA Free
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 600-800 of human SMPD4 (NP_060421.2). ITQAKHTAKSISDQCAESPAGHSFLSWLGFSSMDTNGSYTANDLDEMGQDSVRKTDEYLEKALEYLRQIFRLSEAQLRQFTLALGTTQDENGKKQLPDCIVGEDGLILTPLGRYQIINGLRRFEIEYQGDPELQPIRSYEIASLVRTLFRLSSAINHRFAGQMAALCSRDDFLGSFCRYHLTEPGLASRHLLSPVGRRQVA
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
98 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for SMPD4 Antibody - Azide and BSA Free
![Western Blot: SMPD4 AntibodyAzide and BSA Free [NBP2-93253]](https://resources.bio-techne.com/images/products/SMPD4-Antibody-Western-Blot-NBP2-93253-img0001.jpg "Western Blot: SMPD4 AntibodyAzide and BSA Free [NBP2-93253]")
Western Blot: SMPD4 AntibodyAzide and BSA Free [NBP2-93253]
Western Blot: SMPD4 Antibody [NBP2-93253] - Analysis of extracts of A-549 cells, using SMPD4 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time:![Immunocytochemistry/ Immunofluorescence: SMPD4 Antibody - Azide and BSA Free [NBP2-93253]](https://resources.bio-techne.com/images/products/SMPD4-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-93253-img0002.jpg "Immunocytochemistry/ Immunofluorescence: SMPD4 Antibody - Azide and BSA Free [NBP2-93253]")
Immunocytochemistry/ Immunofluorescence: SMPD4 Antibody - Azide and BSA Free [NBP2-93253]
Immunocytochemistry/Immunofluorescence: SMPD4 Antibody [NBP2-93253] - Analysis of L929 cells using SMPD4 Rabbit pAb at dilution of 1:100. Blue: DAPI for nuclear staining.Applications for SMPD4 Antibody - Azide and BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:50-1:200
Western Blot
1:200-1:2000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.3), 50% glycerol
Format
Azide and BSA Free
Preservative
0.01% Thimerosal
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: SMPD4
Alternate Names
KIAA1418, NET13, Neutral Sphingomyelinase 3, Neutral Sphingomyelinase III, NSMASE3, NSMASE-3, SKNY Protein, Sphingomyelin Phosphodiesterase 4, Sphingomyelin Phosphodiesterase 4, Neutral Membrane (Neutral Sphingomyelinase-3)
Gene Symbol
SMPD4
Additional SMPD4 Products
Product Documents for SMPD4 Antibody - Azide and BSA Free
Product Specific Notices for SMPD4 Antibody - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov