SMURF1 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # H00057154-D01P
Key Product Details
Species Reactivity
Human
Applications
Proximity Ligation Assay, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
SMURF1 (NP_851994.1, 1 a.a. - 731 a.a.) full-length human protein. MSNPGTRRNGSSIKIRLTVLCAKNLAKKDFFRLPDPFAKIVVDGSGQCHSTDTVKNTLDPKWNQHYDLYVGKTDSITISVWNHKKIHKKQGAGFLGCVRLLSNAISRLKDTGYQRLDLCKLNPSDTDAVRGQIVVSLQTRDRIGTGGSVVDCRGLLENEGTVYEDSGPGRPLSCFMEEPAPYTDSTGAAAGGGNCRFVESPSQDQRLQAQRLRNPDVRGSLQTPQNRPHGHQSPELPEGYEQRTTVQGQVYFLHTQTGVSTWHDPRIPRDLNSVNCDELGPLPPGWEVRSTVSGRIYFVDHNNRTTQFTDPRLHHIMNHQCQLKEPSQPLPLPSEGSLEDEELPAQRYERDLVQKLKVLRHELSLQQPQAGHCRIEVSREEIFEESYRQIMKMRPKDLKKRLMVKFRGEEGLDYGGVAREWLYLLCHEMLNPYYGLFQYSTDNIYMLQINPDSSINPDHLSYFHFVGRIMGLAVFHGHYINGGFTVPFYKQLLGKPIQLSDLESVDPELHKSLVWILENDITPVLDHTFCVEHNAFGRILQHELKPNGRNVPVTEENKKEYVRLYVNWRFMRGIEAQFLALQKGFNELIPQHLLKPFDQKELELIIGGLDKIDLNDWKSNTRLKHCVADSNIVRWFWQAVETFDEERRARLLQFVTGSTRVPLQGFKALQGSTGAAGPRLFTIHLIDANTDNLPKAHTCFNRIDIPPYESYEKLYEKLLTAVEETCGFAVE
Specificity
SMURF1 - SMAD specific E3 ubiquitin protein ligase 1,
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for SMURF1 Antibody
Western Blot: SMURF1 Antibody [H00057154-D01P]
Western Blot: SMURF1 Antibody [H00057154-D01P] - Analysis of SMURF1 expression in transfected 293T cell line by SMURF1 polyclonal antibody.Lane 1: SMURF1 transfected lysate(83.40 KDa).Lane 2: Non-transfected lysate.Proximity Ligation Assay: SMURF1 Antibody [H00057154-D01P]
Proximity Ligation Assay: SMURF1 Antibody [H00057154-D01P] - Analysis of protein-protein interactions between SMURF1 and ECSIT. HeLa cells were stained with anti-SMURF1 rabbit purified polyclonal 1:1200 and anti-ECSIT mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).Applications for SMURF1 Antibody
Application
Recommended Usage
Western Blot
1:500
Application Notes
Antibody reactive against Recombinant Protein with GST tag on ELISA and Western Blot and also on transfected lysate in western blot. GST tag alone is used as a negative control.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS (pH 7.4)
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: SMURF1
Long Name
SMAD Ubiquitination Regulatory Factor 1
Alternate Names
EC 6.3.2, KIAA1625
Entrez Gene IDs
57154 (Human)
Gene Symbol
SMURF1
UniProt
Additional SMURF1 Products
Product Documents for SMURF1 Antibody
Product Specific Notices for SMURF1 Antibody
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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