SMURF2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-20427

Novus Biologicals, part of Bio-Techne
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NBP2-20427
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (100%), Rat (100%), Xenopus (95%), Zebrafish (96%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all SMURF2 Antibodies »

Product Summary for SMURF2 Antibody

Immunogen

Recombinant protein encompassing a sequence within the center region of human SMURF2. The exact sequence is proprietary.

Reactivity Notes

Xenopus laevis (95%).

Localization

Nucleus; Cytoplasm; Cell membrane; Membrane raft

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

86 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for SMURF2 Antibody

Western Blot: SMURF2 Antibody [NBP2-20427]

Western Blot: SMURF2 Antibody [NBP2-20427]

Western Blot: SMURF2 Antibody [NBP2-20427] - Sample (30 ug of whole cell lysate) A: MCF-7 7. 5% SDS PAGE gel, diluted at 1:500.
Immunohistochemistry-Paraffin: SMURF2 Antibody [NBP2-20427]

Immunohistochemistry-Paraffin: SMURF2 Antibody [NBP2-20427]

Immunohistochemistry-Paraffin: SMURF2 Antibody [NBP2-20427] - Human colon cancer. SMURF2 stained by SMURF2 antibody [N3C2], Internal diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Immunohistochemistry-Paraffin: SMURF2 Antibody [NBP2-20427]

Immunohistochemistry-Paraffin: SMURF2 Antibody [NBP2-20427]

Immunohistochemistry-Paraffin: SMURF2 Antibody [NBP2-20427] - Human gastric cancer, using SMURF2 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Applications for SMURF2 Antibody

Application
Recommended Usage

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris (pH 7), 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: SMURF2

Smad ubiquitination regulatory factor proteins (Smurf1 and Smurf2) are E3 ubiquitin ligase that belongs to the Hect family. Smurf proteins play an important role as regulators in TGF-beta pathway by ubiquitinating Smads and Smads associated proteins for proteasome degradation (1). Specifically, Smurf1 interacts with Smad1 and Smad5 for degradation, while Smurf2 ubiquitinates Smad1 and Smad2. Smads also functions to recruit Smurfs to various pathway components such as TGF-beta and SnoN. In particular, Smad7 acts as an adaptor protein between Smurfs and TGF-Beta receptors, allowing the receptors to be marked by Smurfs for degradation (2). Smurf2 interacts with all members of Smad family except for Smad4 and it is expressed various tissues and cell lines, such as placenta and ovarian cancer cell lines. Smurf2 has been implicated in the tumor formation and diseases progression (3).

Long Name

SMAD Ubiquitination Regulatory Factor 2

Alternate Names

DKFZp686F0270, E3 ubiquitin ligase SMURF2, E3 ubiquitin-protein ligase SMURF2, EC 6.3.2, EC 6.3.2.-, hSMURF2, MGC138150, SMAD specific E3 ubiquitin protein ligase 2, SMAD ubiquitination regulatory factor 2, SMAD-specific E3 ubiquitin-protein ligase 2

Gene Symbol

SMURF2

UniProt

Q9HAU4

Additional SMURF2 Products

Product Documents for SMURF2 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for SMURF2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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