SMYD3 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-20430

Novus Biologicals, part of Bio-Techne
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NBP2-20430
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Predicted:

Monkey (99%), Porcine (92%). Backed by our 100% Guarantee.

Applications

Chromatin Immunoprecipitation (ChIP), Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all SMYD3 Antibodies »

Product Summary for SMYD3 Antibody

Immunogen

Recombinant protein encompassing a sequence within the center region of human SMYD3. The exact sequence is proprietary.

Reactivity Notes

Rhesus Monkey (99%), Bovine (80%).

Localization

Cytoplasm; Nucleus

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

49 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for SMYD3 Antibody

Western Blot: SMYD3 Antibody [NBP2-20430]

Western Blot: SMYD3 Antibody [NBP2-20430]

Western Blot: SMYD3 Antibody [NBP2-20430] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with SMYD3 antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Immunocytochemistry/ Immunofluorescence: SMYD3 Antibody [NBP2-20430]

Immunocytochemistry/ Immunofluorescence: SMYD3 Antibody [NBP2-20430]

Immunocytochemistry/Immunofluorescence: SMYD3 Antibody [NBP2-20430] - HCT116 cells were fixed in ice-cold MeOH for 5 min. Green: SMYD3 protein stained by SMYD3 antibody diluted at 1:500.Blue: Hoechst 33342 staining.
Chromatin Immunoprecipitation: SMYD3 Antibody [NBP2-20430] - Cross-linked ChIP was performed with HepG2 chromatin extract and 5 ug of either normal rabbit IgG or anti-SMYD3 antibody. The precipitated DNA was detected by PCR with primer set targeting to 5' flanking region of Nkx2.8..
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Applications for SMYD3 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunoprecipitation

1:100-1:500

Western Blot

1:500-1:3000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS (pH 7), 20% Glycerol

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: SMYD3

SMYD3 is a histone methyltransferase that plays a role in transcriptional regulation as a member of an RNA polymerase complex.[supplied by OMIM]

Long Name

Histone-lysine N-methyltransferase SMYD3

Alternate Names

ZMYND1, ZNFN3A1

Gene Symbol

SMYD3

Additional SMYD3 Products

Product Documents for SMYD3 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for SMYD3 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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