SMYD4 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-81356
Key Product Details
Species Reactivity
Human
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: ACQTEAHRMAAGPRWEAFCCNSCGAPMQGDDVLRCGSRSCAESAVSRDHLVSRLQDLQQQVRVAQKLLRDGELERAVQRLSGCQR
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for SMYD4 Antibody
![Western Blot: SMYD4 Antibody [NBP1-81356]](https://resources.bio-techne.com/images/products/SMYD4-Antibody-Western-Blot-NBP1-81356-img0010.jpg "Western Blot: SMYD4 Antibody [NBP1-81356]")
Western Blot: SMYD4 Antibody [NBP1-81356]
Western Blot: SMYD4 Antibody [NBP1-81356] - Analysis in control (vector only transfected HEK293T lysate) and SMYD4 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).![Immunocytochemistry/ Immunofluorescence: SMYD4 Antibody [NBP1-81356]](https://resources.bio-techne.com/images/products/SMYD4-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-81356-img0009.jpg "Immunocytochemistry/ Immunofluorescence: SMYD4 Antibody [NBP1-81356]")
Immunocytochemistry/ Immunofluorescence: SMYD4 Antibody [NBP1-81356]
Immunocytochemistry/Immunofluorescence: SMYD4 Antibody [NBP1-81356] - Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm, the Golgi apparatus & vesicles.![Immunohistochemistry-Paraffin: SMYD4 Antibody [NBP1-81356]](https://resources.bio-techne.com/images/products/SMYD4-Antibody-Immunohistochemistry-Paraffin-NBP1-81356-img0008.jpg "Immunohistochemistry-Paraffin: SMYD4 Antibody [NBP1-81356]")
Immunohistochemistry-Paraffin: SMYD4 Antibody [NBP1-81356]
Immunohistochemistry-Paraffin: SMYD4 Antibody [NBP1-81356] - Staining of human ovary shows cytoplasmic positivity in follicle cells.Applications for SMYD4 Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
0.25-2 ug/ml
Immunohistochemistry
1:50 - 1:200
Immunohistochemistry-Paraffin
1:50 - 1:200
Western Blot
0.04 - 0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: SMYD4
Alternate Names
KIAA1936ZMYND21, SET and MYND domain containing 4, SET and MYND domain-containing protein 4
Entrez Gene IDs
114826 (Human)
Gene Symbol
SMYD4
UniProt
Additional SMYD4 Products
Product Documents for SMYD4 Antibody
Product Specific Notices for SMYD4 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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