SNAP23 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-49855

Novus Biologicals, part of Bio-Techne
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NBP1-49855
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all SNAP23 Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-QIKRITDKAD corresponding to internal region according to NP_570710.1.

Epitope

QIKRITDKAD - internal region

Reactivity Notes

Expected from sequence similarity: Mouse, Rat, Bovine

Specificity

This antibody is expected to recognize both reported isoforms (NP_003816.2; NP_570710.1).

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for SNAP23 Antibody

Western Blot: SNAP23 Antibody [NBP1-49855]

Western Blot: SNAP23 Antibody [NBP1-49855]

Western Blot: SNAP23 Antibody [NBP1-49855] - Analysis of SNAP23 in Human Placenta lysate (35ug protein in RIPA buffer) using this antibody at 0.1 ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: SNAP23 Antibody [NBP1-49855]

Immunohistochemistry-Paraffin: SNAP23 Antibody [NBP1-49855]

Immunohistochemistry-Paraffin: SNAP23 Antibody [NBP1-49855] - 3.75ug/ml staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Applications for SNAP23 Antibody

Application
Recommended Usage

Immunohistochemistry

0.1-0.3ug/ml

Peptide ELISA

Detection limit 1:8000

Western Blot

0.1 ug/ml
Application Notes
Western blot: Approx 26kDa band observed in Human Placenta lysates (calculated MW of 23.4kDa according to NP_003816.2). The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Primary incubation was 1 hour
IHC: Paraffin embedded Spleen.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: SNAP23

Synaptosomal-associated proteins (SNAPs) are cytosolic proteins that play a key role in in the process of membrane fusion in intracellular vesicle trafficking. In eukaryotic cells, the SNARE (soluble N-ethylmaleimide-sensitive factor attachment protein receptor) complex is critical to membrane docking and fusion and is believed to impart some degree of specificity between vesicle SNARE (v-SNARE) and target organelle SNARE (t-SNARE). In neurons and neuroendocrine cells, the SNARE complex consists of the integral membrane proteins VAMP (vesicle-associated membrane protein), syntaxin and SNAP-25. In non-neuronal tissue, a SNAP-25 homolog, SNAP-23, functionally replaces SNAP-25 in the SNARE complex. Studies show that VAMP, syntaxin and SNAP-23 are required for SNARE function and that this complex exists as a heterotrimer of the three proteins. In insulin signalling pathways, studies suggest that the translocation of GLUT4 in adipocytes requires that functional SNAP-23 be present in SNARE complexes.

Long Name

Synaptosomal-associated Protein, 23kDa

Alternate Names

HsT17016, SNAP-23, SNAP23A, SNAP23B, synaptosomal-associated protein 23, synaptosomal-associated protein, 23kD, synaptosomal-associated protein, 23kDa, Vesicle-membrane fusion protein SNAP-23

Entrez Gene IDs

8773 (Human); 20619 (Mouse); 64630 (Rat)

Gene Symbol

SNAP23

UniProt

NP_003816.2 (Human)

Additional SNAP23 Products

Product Documents for SNAP23 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for SNAP23 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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