SNF5 Antibody (9A9G0)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16159

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-16159-100ul
NBP3-16159-20ul
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Knockout Validated, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 9A9G0

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all SNF5 Antibodies »

Product Summary for SNF5 Antibody (9A9G0)

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 50-150 of human SNF5 (NP_003064.2). LWRRLATVEERKKIVASSHGKKTKPNTKDHGYTTLATSVTLLKASEVEEILDGNDEKYKAVSISTEPPTYLREQKAKRNSQWVPTLPNSSHHLDAVPCSTT

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SNF5 Antibody (9A9G0)

Western Blot: SNF5 Antibody (9A9G0) [NBP3-16159]

Western Blot: SNF5 Antibody (9A9G0) [NBP3-16159]

Western Blot: SNF5 Antibody (6X6T8) [NBP3-16159] - Western blot analysis of extracts of various cell lines, using SMARCB1/SNF5 Rabbit mAb (NBP3-16159) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.
Immunocytochemistry/ Immunofluorescence: SNF5 Antibody (9A9G0) [NBP3-16159]

Immunocytochemistry/ Immunofluorescence: SNF5 Antibody (9A9G0) [NBP3-16159]

Immunocytochemistry/Immunofluorescence: SNF5 Antibody (6X6T8) [NBP3-16159] - Immunofluorescence analysis of U-2 OS cells using SMARCB1/SNF5 Rabbit mAb (NBP3-16159) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Western Blot: SNF5 Antibody (9A9G0) [NBP3-16159]

Western Blot: SNF5 Antibody (9A9G0) [NBP3-16159]

Western Blot: SNF5 Antibody (6X6T8) [NBP3-16159] - Western blot analysis of extracts of various cell lines, using SMARCB1/SNF5 Rabbit mAb (NBP3-16159) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
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Applications for SNF5 Antibody (9A9G0)

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

1:1000 - 1:5000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.05% Proclin 300

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: SNF5

SNF5 is encoded by this gene is part of a complex that relieves repressive chromatin structures, allowing the transcriptional machinery to access its targets more effectively. The encoded nuclear protein may also bind to and enhance the DNA joining activity of HIV-1 integrase. This gene has been found to be a tumor suppressor, and mutations in it have been associated with malignant rhabdoid tumors. Two transcript variants encoding different isoforms have been found for this gene.

Alternate Names

BAF47, BAF47hSNF5, BRG1-associated factor 47, hSNFS, Ini1, INI1RTPS1, Integrase interactor 1 protein, malignant rhabdoid tumor suppressor, RDTSNF5 homolog, Sfh1p, SNF5, SNF5L1, Snr1, subfamily b, member 1, sucrose nonfermenting, yeast, homolog-like 1, SWI/SNF related, matrix associated, actin dependent regulator of chromatin, SWI/SNF-related matrix-associated actin-dependent regulator of chromatinsubfamily B member 1

Gene Symbol

SMARCB1

Additional SNF5 Products

Product Documents for SNF5 Antibody (9A9G0)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for SNF5 Antibody (9A9G0)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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