SNF5 Antibody (CL14031)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-24595
Key Product Details
Species Reactivity
Validated:
Human
Predicted:
Mouse (100%), Rat (100%). Backed by our 100% Guarantee.
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # CL14031
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Summary for SNF5 Antibody (CL14031)
Immunogen
This antibody was designed to target the recombinant protein SNF5, with the precise immunogen sequence being withheld for proprietary reasons.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Scientific Data Images for SNF5 Antibody (CL14031)
Immunohistochemistry-Paraffin: SNF5 Antibody (CL14031) [NBP3-24595]
Staining of human lymph node shows strong nuclear positivity in non-germinal and germinal center cells.
Immunohistochemistry-Paraffin: SNF5 Antibody (CL14031) [NBP3-24595]
Staining of human ovarian cancer (papillary carcinoma) shows strong nuclear positivity in tumor cells.
Immunocytochemistry/Immunofluorescence: SNF5 Antibody (CL14031) [NBP3-24595]
Staining in RH-30 cell line with Anti-SMARCB1 monoclonal antibody, showing specific staining of nucleoplasm in green. Microtubule- and nuclear probes are visualized in red and blue respectively (where available).
Applications for SNF5 Antibody (CL14031)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
2-10 ug/ml
Immunohistochemistry
1:1000-1:2500
Immunohistochemistry-Paraffin
1:1000-1:2500
Western Blot
1ug/ml
Application Notes
For IHC-Paraffin, we recommend using Heat-Induced Epitope Retrieval (HIER) with a pH level of 6. This optimized retrieval method ensures the best results for your experiments, For ICC/IF, we recommend using a combination of PFA and Triton X-100. This will give you the optimal results for your experiments.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS, pH 7.2, 40% glycerol
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: SNF5
Alternate Names
BAF47, BAF47hSNF5, BRG1-associated factor 47, hSNFS, Ini1, INI1RTPS1, Integrase interactor 1 protein, malignant rhabdoid tumor suppressor, RDTSNF5 homolog, Sfh1p, SNF5, SNF5L1, Snr1, subfamily b, member 1, sucrose nonfermenting, yeast, homolog-like 1, SWI/SNF related, matrix associated, actin dependent regulator of chromatin, SWI/SNF-related matrix-associated actin-dependent regulator of chromatinsubfamily B member 1
Gene Symbol
SMARCB1
Additional SNF5 Products
Product Documents for SNF5 Antibody (CL14031)
Product Specific Notices for SNF5 Antibody (CL14031)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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