SNF8 Antibody (6B11)

Novus Biologicals, part of Bio-Techne | Catalog # H00011267-M01

Novus Biologicals, part of Bio-Techne
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H00011267-M01
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 6B11

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all SNF8 Antibodies »

Product Specifications

Immunogen

SNF8 (NP_009172, 23 a.a. ~ 122 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. ERGTVLAEDQLAQMSKQLDMFKTNLEEFASKHKQEIRKNPEFRVQFQDMCATIGVDPLASGKGFWSEMLGVGDFYYELGVQIIEVCLALKHRNGGLITLE

Reactivity Notes

Human. Other species not tested.

Specificity

SNF8 - EAP30 subunit of ELL complex

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for SNF8 Antibody (6B11)

Western Blot: SNF8 Antibody (6B11) [H00011267-M01]

Western Blot: SNF8 Antibody (6B11) [H00011267-M01]

Western Blot: SNF8 Antibody (6B11) [H00011267-M01] - Analysis of SNF8 expression in transfected 293T cell line by SNF8 monoclonal antibody (M01), clone 6B11.Lane 1: SNF8 transfected lysate (Predicted MW: 28.9 KDa).Lane 2: Non-transfected lysate.
Western Blot: SNF8 Antibody (6B11) [H00011267-M01]

Western Blot: SNF8 Antibody (6B11) [H00011267-M01]

Western Blot: SNF8 Antibody (6B11) [H00011267-M01] - EAP30 monoclonal antibody (M01), clone 6B11 Western Blot analysis of SNF8 expression in HeLa NE ( Cat # L013V3 ).
Sandwich ELISA: SNF8 Antibody (6B11) [H00011267-M01] - Detection limit for recombinant GST tagged SNF8 is approximately 1ng/ml as a capture antibody.

Applications for SNF8 Antibody (6B11)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactive against cell lysate and recombinant protein for western blot. It has also been used for ELISA.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: SNF8

SNF8, VPS25 (MIM 610907), and VPS36 (MIM 610903) form ESCRT-II (endosomal sorting complex required for transport II), a complex involved in endocytosis of ubiquitinated membrane proteins. SNF8, VPS25, and VPS36 are also associated in a multiprotein complex with RNA polymerase II elongation factor (ELL; MIM 600284) (Slagsvold et al., 2005 [PubMed 15755741]; Kamura et al., 2001 [PubMed 11278625]).[supplied by OMIM]

Alternate Names

EAP30 subunit of ELL complex, EAP30hVps22, ELL-associated protein of 30 kDa, ESCRT-II complex subunit VPS22, SNF8, ESCRT-II complex subunit, homolog (S. cerevisiae), vacuolar-sorting protein SNF8, VPS22Dot3

Entrez Gene IDs

11267 (Human)

Gene Symbol

SNF8

UniProt

NP_009172 (Human)

Additional SNF8 Products

Product Documents for SNF8 Antibody (6B11)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for SNF8 Antibody (6B11)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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