Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI3E2

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all SnoN/Ski-like Protein Antibodies »

Product Specifications

Immunogen

Human recombinant protein fragment corresponding to amino acids 307-684 of human SKIL (NP_005405) produced in E.coli.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

76.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for SnoN Antibody (OTI3E2) - Azide and BSA Free

Western Blot: SnoN Antibody (OTI3E2)Azide and BSA Free [NBP2-74255]

Western Blot: SnoN Antibody (OTI3E2)Azide and BSA Free [NBP2-74255]

Western Blot: SnoN Antibody (OTI3E2) - Azide and BSA Free [NBP2-74255] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SnoN (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SnoN.
Immunohistochemistry: SnoN Antibody (OTI3E2) - Azide and BSA Free [NBP2-74255]

Immunohistochemistry: SnoN Antibody (OTI3E2) - Azide and BSA Free [NBP2-74255]

Immunohistochemistry: SnoN Antibody (OTI3E2) - Azide and BSA Free [NBP2-74255] - Staining of paraffin-embedded Human liver tissue using anti-SnoN mouse monoclonal antibody.
Immunohistochemistry: SnoN Antibody (OTI3E2) - Azide and BSA Free [NBP2-74255]

Immunohistochemistry: SnoN Antibody (OTI3E2) - Azide and BSA Free [NBP2-74255]

Immunohistochemistry: SnoN Antibody (OTI3E2) - Azide and BSA Free [NBP2-74255] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-SnoN mouse monoclonal antibody.
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Applications for SnoN Antibody (OTI3E2) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry

1:150

Western Blot

1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: SnoN/Ski-like Protein

SnoN may have regulatory role in cell division or differentiation in response to extracellular signals

Long Name

SKI-like Oncogene

Alternate Names

Ski like Protein, SKIL, SNO, SnoA, SnoI

Gene Symbol

SKIL

Additional SnoN/Ski-like Protein Products

Product Documents for SnoN Antibody (OTI3E2) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for SnoN Antibody (OTI3E2) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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