SNX16 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-1211

Novus Biologicals, part of Bio-Techne
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NB100-1211
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Bovine (100%), Canine (100%), Mouse (100%), Porcine (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all SNX16 Antibodies »

Product Specifications

Immunogen

Peptide with sequence ATPYVPVPMPIGN-C corresponding to N-Terminus according to NP_690049.1, NP_690050.1.

Specificity

This antibody is expected to recognise both isoform a (NP_071416.2; NP_690049.1) and isoform b (NP_690050.1).

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for SNX16 Antibody

Western Blot: SNX16 Antibody [NB100-1211]

Western Blot: SNX16 Antibody [NB100-1211]

Western Blot: SNX16 Antibody [NB100-1211] - HEK293 overexpressing SNX16 and probed with (mock transfection in first lane).
Immunohistochemistry-Paraffin: SNX16 Antibody [NB100-1211]

Immunohistochemistry-Paraffin: SNX16 Antibody [NB100-1211]

Immunohistochemistry-Paraffin: SNX16 Antibody [NB100-1211] - Staining of paraffin embedded Human Breast. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Western Blot: SNX16 Antibody [NB100-1211]

Western Blot: SNX16 Antibody [NB100-1211]

Western Blot: SNX16 Antibody [NB100-1211] - (0.05ug/ml) staining of Daudi lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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Applications for SNX16 Antibody

Application
Recommended Usage

Immunohistochemistry

3.75 ug/ml

Immunohistochemistry-Paraffin

3.75 ug/ml

Peptide ELISA

Detection limit 1:64000

Western Blot

0.05 - 0.2 ug/ml
Application Notes
WB: Approx. 38 kDa band observed in Daudi lysates (calculated MW of 39.2 kDa band according to NP_071416.2). In transfected HEK293 transiently expressing SNX16 a band of approx. 40 kDa is observed. This band is not observed in the non-transfected HEK293.
IHC: Paraffin embedded Human Placenta, Tonsil and Breast.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: SNX16

This gene encodes a member of the sorting nexin family. Members of this family contain a phox (PX) domain, which is a phosphoinositide binding domain, and are involved in intracellular trafficking. The function of this protein has not been determined. This gene results in three transcript variants encoding two distinct isoforms.

Alternate Names

DKFZp666H147, sorting nexin 16, sorting nexin-16

Entrez Gene IDs

64089 (Human); 74718 (Mouse); 64088 (Rat)

Gene Symbol

SNX16

UniProt

NP_071416 (Human)

Additional SNX16 Products

Product Documents for SNX16 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for SNX16 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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