SNX4 Antibody (JE65-39)

Western Blot: SNX4 Antibody (JE65-39) [NBP3-32964]

Western Blot: SNX4 Antibody (JE65-39) [NBP3-32964] - Western blot analysis of SNX4 on different lysates with Rabbit anti-SNX4 antibody (NBP3-32964) at 1/1,000 dilution.

Lane 1: HEK-293 cell lysate
Lane 2: HeLa cell lysate
Lane 3: Jurkat cell lysate
Lane 4: A431 cell lysate
Lane 5: RAW264.7 cell lysate
Lane 6: PC-12 cell lysate

Lysates/proteins at 20 ug/Lane.

Predicted band size: 52 kDa
Observed band size: 52/36 kDa

Exposure time: 1 minute;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32964) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Anti-Rabbit IgG for IP Nano-secondary antibody (NBI01H) at 1/5,000 dilution was used for 1 hour at room temperature.