SOCS-7/Nck/NAP4 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-06994

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all SOCS-7/Nck/NAP4 Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-DAGRKPKLTRTQS corresponding to internal region according to NP_055413.1.

Epitope

internal region

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for SOCS-7/Nck/NAP4 Antibody

Western Blot: SOCS-7/Nck/NAP4 Antibody [NBP1-06994]

Western Blot: SOCS-7/Nck/NAP4 Antibody [NBP1-06994]

Western Blot: SOCS-7/Nck/NAP4 Antibody [NBP1-06994] - Analysis of SOCS7-7/Nck/NAP4 in Daudi lysate (35ug protein in RIPA buffer) using NBP1-06994 at 1ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence.

Applications for SOCS-7/Nck/NAP4 Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:32000

Western Blot

1 - 3 ug/ml
Application Notes
WB: Approx. 60 kDa band observed in lysates of Daudi (calculated MW of 63.0 kDa band according to NP_055413.1). An additional band of unknown identity was also consistently observed at 28 kDa. This band was successfully blocked by incubation with the immunizing peptide.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: SOCS-7/Nck/NAP4

Regulates signaling cascades probably through protein ubiquitination and/or sequestration. Functions in insulin signaling and glucose homeostasis through IRS1 ubiquitination and subsequent proteasomal degradation. Inhibits also prolactin, growth hormone and leptin signaling by preventing STAT3 and STAT5 activation, sequestering them in the cytoplasm and reducing their binding to DNA. May be a substrate recognition component of a SCF-like E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins

Long Name

Suppressor Of Cytokine Signaling 7

Alternate Names

NAP4, Nck, SOCS7

Entrez Gene IDs

30837 (Human)

Gene Symbol

SOCS7

UniProt

NP_055413.1 (Human)

Additional SOCS-7/Nck/NAP4 Products

Product Documents for SOCS-7/Nck/NAP4 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for SOCS-7/Nck/NAP4 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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