SOD2/Mn-SOD Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-1969

Novus Biologicals, part of Bio-Techne
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NB100-1969-25ug
NB100-1969
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Porcine, Bovine, Canine, Chicken, Gerbil, Guinea Pig, Hamster, Monkey, Rabbit, Sheep, Xenopus

Cited:

Mouse

Applications

Validated:

ELISA, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Cited:

Immunohistochemistry-Frozen

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1 mg/ml

View all SOD2/Mn-SOD Antibodies »

Product Specifications

Immunogen

Recombinant Human Mn SOD Protein

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 24578415). Immunogen displays sequence identity for non-tested species: Rat, Bovine, Canine, Chicken, Gerbil, Guinea pig, Porcine, Hamster, Monkey, Rabbit, Sheep, Xenopus, Frog and Wood Frog (Rana sylvatica).

Localization

Mitochondria, Mitochondria Matrix

Specificity

Detects approx 25 kDa.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SOD2/Mn-SOD Antibody

Western Blot: SOD2/Mn-SOD Antibody [NB100-1969]

Western Blot: SOD2/Mn-SOD Antibody [NB100-1969]

Western Blot: SOD2/Mn-SOD Antibody [NB100-1969] - Western blot analysis of Mouse Brain, Rat Brain, and Rat Brain Membrane showing detection of 24.7kDa SOD (Mn) protein using Rabbit Anti-SOD (Mn) Polyclonal Antibody (NB100-1969). Lane 1: MW Ladder. Lane 2: Mouse Brain (15 ug). Lane 3: Rat Brain (15 ug). Lane 4: Rat Brain Membrane (15 ug). Block: 5% Skim Milk powder in TBST. Primary Antibody: Rabbit Anti-SOD (Mn) Polyclonal Antibody (NB100-1969) at 1:1000 for O/N at 4C. Secondary Antibody: Goat anti-rabbit IgG:HRP at 1:5000 for 1 hour at RT with shaking. Color Development: Chemiluminescent for HRP (Moss) for 5 min in RT. Predicted/Observed Size: 24.7kDa.

Applications for SOD2/Mn-SOD Antibody

Application
Recommended Usage

ELISA

1:100 - 1:2000

Immunohistochemistry

1:100

Immunohistochemistry-Frozen

1:100

Immunohistochemistry-Paraffin

1:100

Immunoprecipitation

1:10 - 1:500

Western Blot

1:5000
Application Notes
0.2 ug/mL of SOD (Mn) Antibody was sufficient for detection of Mn SOD in 20 ug of rat brain tissue extract by colorimetric immunoblot analysis using Goat anti-mouse IgG:AP as the secondary Antibody. Use in Immunohistochemistry-Frozen reported in scientific literature (PMID 24578415).

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.4), 50% Glycerol

Preservative

0.09% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SOD2/Mn-SOD

Superoxide dismutase (SOD) is an endogenously produced intracellular enzyme present in almost every cell in the body (3). It works by catalyzing the dismutation of the superoxide radical O2- to O2 and H2O2, which are then metabolized to H2O and O2 by catalase and glutathione peroxidase (2,5). In general, SODs play a major role in antioxidant defense mechanisms (4). There are two main types of SOD in mammalian cells. One form (SOD1) contains Cu and Zn ions as a homodimer and exists in the cytoplasm. The two subunits of 16 kDa each are linked by two cysteines forming an intra-subunit disulphide bridge (3). The second form (SOD2) is a manganese containing enzyme and resides in the mitochondrial matrix. It is a homotetramer of 80 kDa. The third form (SOD3 or EC-SOD) is like SOD1 in that it contains Cu and Zn ions, however it is distinct in that it is a homotetramer, with a mass of 30 kDA and it exists only in the extracellular space(8). SOD3 can also be distinguished by its heparin-binding capacity (1).

Long Name

Superoxide Dismutase-2

Alternate Names

IPO-B, Mn SOD, MnSOD, SOD, Mitochodrial

Entrez Gene IDs

6648 (Human); 20656 (Mouse); 24787 (Rat)

Gene Symbol

SOD2

UniProt

P04179

Additional SOD2/Mn-SOD Products

Product Documents for SOD2/Mn-SOD Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for SOD2/Mn-SOD Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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