SODD/BAG4 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-27329

Novus Biologicals, part of Bio-Techne
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NBP2-27329SS
NBP2-27329
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Cited:

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

View all SODD/BAG4 Antibodies »

Product Specifications

Immunogen

This antibody was raised against BAG-4

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SODD/BAG4 Antibody

Immunohistochemistry-Paraffin: SODD/BAG4 Antibody [NBP2-27329]

Immunohistochemistry-Paraffin: SODD/BAG4 Antibody [NBP2-27329]

Immunohistochemistry-Paraffin: SODD/BAG4 Antibody [NBP2-27329] - IHC analysis of formalin fixed paraffin-embedded (FFPE) human breast cancer using SODD/BAG4 antibody at 1:5000 on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images was performed using Aperio AT2 (Leica Biosystems). Connective tissue staining was observed. Staining was performed by Histowiz.

Applications for SODD/BAG4 Antibody

Application
Recommended Usage

Immunohistochemistry-Frozen

1:1000-1:5000

Immunohistochemistry-Paraffin

1:1000-1:5000~

Immunoprecipitation

1:50-1:200

Western Blot

1:1000-1:2000~

Formulation, Preparation, and Storage

Purification

Unpurified

Formulation

50 ul neat whole antisera

Preservative

0.05% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SODD/BAG4

The BAG proteins are a family of chaperone regulators that modulate a number of diverse processes including proliferation, survival, stress responses, tumorigenesis, neuronal differentiation, growth arrest and apoptosis (reviewed Takayama and Reed, 2001; Doong et al, 2002, and Doukhanina et al. 2006). BAG proteins have been characterized as co-chaperones and interact with the chaperone heat shock proteins 70, both constitutive Hsc70 and inducible Hsp70. BAG proteins bind through their BAG domain to the ATPase domain of Hsc70/Hsp70, and can modulate either positively or negatively the functions of the Hsc70/Hsp70 chaperone proteins. The BAG domain has been shown to contribute to the anti-apoptotic activity of BAG- family proteins. The anti-apoptotic activities of BAG-family proteins may be dependent on their interactions with Hsc70/Asp70 and/or binding to Bcl-2. In addition to the conserved BAG domain, BAG-family proteins also contain additional domains which enable them to interact with specific target proteins or to target them to specific locations within cells. The BAG family contains at least six family members, including BAG-1 and its various isoforms [including BAG-1S , BAG-1M (RAP46/HAP46), and BAG-1L, BAG2, BAG3 (CAIR-1; Bis,), BAG4 (SODD), BAG5 and BAG6 (Scythe, BAT3). The following amino acids (aa) lengths and molecular weights (kDa) have been described for human BAG proteins (reviewed in Takayama et al, 2001 and Doong et al, 2002): BAG-1 (230 aa., 34 kDa), BAG-1S (219 aa, 29 kDa), BAG-1M (274 aa, 46 kDa), BAG-1L (345 aa, 52 kDa), BAG-2 [212 aa; 24 kDa (Arndt et al. 2005)], BAG-3 (575 aa, 74 kDa), BAG-4 (456 aa; 60 kDa), BAG-5 ([442 aa; 51 kDa (kalia et al. 2004)], and BAG-6 (1129 aa; 150 kDa). IMG-5668 recognizes both BAG-4.

Long Name

Silencer of Death Domains

Alternate Names

BAG4

Gene Symbol

BAG4

UniProt

O95429

Additional SODD/BAG4 Products

Product Documents for SODD/BAG4 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for SODD/BAG4 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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