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Sodium Potassium ATPase Beta 1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-54665

Novus Biologicals, part of Bio-Techne
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NBP2-54665
NBP2-54665-25ul

Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Predicted:

Mouse (93%), Rat (93%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Summary for Sodium Potassium ATPase Beta 1 Antibody

Immunogen

This antibody was developed against a Recombinant Protein corresponding to amino acids: SEFKPTYQDRVAPPGLTQIPQIQKTEISFRPNDPKSYEAYVLNIVRFLEKYKDSAQRDDMIFEDCGDVPSEPKERGDFNHERGERKVCRFKLEWLGNCSGLNDETYGYKEGKPCIIIKLNRVLGFKPKPPKNESLETYPVMKYNPN

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Sodium Potassium ATPase Beta 1 Antibody

Immunohistochemistry-Paraffin: Sodium Potassium ATPase Beta 1 Antibody [NBP2-54665]

Immunohistochemistry-Paraffin: Sodium Potassium ATPase Beta 1 Antibody [NBP2-54665]

Immunohistochemistry-Paraffin: Sodium Potassium ATPase Beta 1 Antibody [NBP2-54665] - Staining in human kidney and lymph node tissues using anti-ATP1B1 antibody. Corresponding ATP1B1 RNA-seq data are presented for the same tissues.
Western Blot: Sodium Potassium ATPase Beta 1 Antibody [NBP2-54665]

Western Blot: Sodium Potassium ATPase Beta 1 Antibody [NBP2-54665]

Western Blot: Sodium Potassium ATPase Beta 1 Antibody [NBP2-54665] - Analysis in human cell lines Caco-2 and SK-MEL-30 using anti-ATP1B1 antibody. Corresponding ATP1B1 RNA-seq data are presented for the same cell lines. Loading control: anti-HDAC1.
Immunohistochemistry-Paraffin: Sodium Potassium ATPase Beta 1 Antibody [NBP2-54665]

Immunohistochemistry-Paraffin: Sodium Potassium ATPase Beta 1 Antibody [NBP2-54665]

Immunohistochemistry-Paraffin: Sodium Potassium ATPase Beta 1 Antibody [NBP2-54665] - Staining of human kidney shows high expression.

Applications for Sodium Potassium ATPase Beta 1 Antibody

Application
Recommended Usage

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Sodium Potassium ATPase Beta 1

Sodium Potassium ATPase Beta 1 (ATP1B1) is an integral membrane protein complex that hydrolyzes ATP to maintain the transmembrane gradients of Na+ and K+ found in most mammalian cells. Na/K ATPase pumps consist of alpha/beta heterodimers. Beta 1 is the glycosylated, non-catalytic component of the active enzyme responsible for the formation of these alpha/beta heterodimers. Through this assembly, the beta polypeptide regulates the number of sodium pumps transported to the plasma membrane.

Long Name

Sodium/potassium-transporting ATPase subunit beta-1

Alternate Names

ATP1B, ATP1B1

Gene Symbol

ATP1B1

Additional Sodium Potassium ATPase Beta 1 Products

Product Documents for Sodium Potassium ATPase Beta 1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Sodium Potassium ATPase Beta 1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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