Sorbitol Dehydrogenase Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-1486

Novus Biologicals, part of Bio-Techne
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NB100-1486
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Predicted:

Canine (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Validated:

Peptide ELISA, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all Sorbitol Dehydrogenase Antibodies »

Product Specifications

Immunogen

Peptide with sequence KIMLKCDPSDQNP corresponding to C-Terminus according to NP_003095.2.

Reactivity Notes

Human reactivity reported in scientific literature (PMID: 30651350).

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Sorbitol Dehydrogenase Antibody

Western Blot: Sorbitol Dehydrogenase Antibody [NB100-1486]

Western Blot: Sorbitol Dehydrogenase Antibody [NB100-1486]

Western Blot: Sorbitol Dehydrogenase Antibody [NB100-1486] - Staining of mouse kidney lysate (35 ug protein in RIPA buffer). Antibody at 0.1 ug/mL. Detected by chemiluminescence.

Applications for Sorbitol Dehydrogenase Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:16000

Western Blot

0.1 - 0.3 ug/mL
Application Notes
WB: Approx. 38 kDa band observed in mouse kidney lysates (calculated MW of 38.3 kDa band according to NP_003095.1). A minor band of unknown identity was also consistently observed at 48 kDa. This band was successfully blocked by incubation with the immunizing peptide.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Sorbitol Dehydrogenase

Sorbitol dehydrogenase (SDH), a member of the medium-chain dehydrogenase/reductase protein family and the second enzyme of the polyol pathway of glucose metabolism, converts sorbitol to fructose strictly using NAD(+) as coenzyme. Sorbitol dehydrogenase catalyzes the interconversion of polyols and their corresponding ketoses, and together with aldose reductase makes up the sorbitol pathway that is believed to play an important role in the development of diabetic complications. The first reaction of the pathway (also called the polyol pathway) is the reduction of glucose to sorbitol by ALDR1 with NADPH as the cofactor. SORD then oxidizes the sorbitol to fructose using NAD(+) cofactor. It has been noted that the polyol pathway is particularly active in hyperglycemic states. Although SORD is closely related to the class I long-chain alcohol dehydrogenases, it differs in substrate specificity, catalyzing polyols such as sorbitol and xylitol but having no activity towards primary alcohols.

Alternate Names

EC 1.1.1, EC 1.1.1.14, L-iditol 2-dehydrogenase, sorbitol dehydrogenase, SORD1

Entrez Gene IDs

6652 (Human); 20322 (Mouse)

Gene Symbol

SORD

UniProt

NP_003095 (Human)

Additional Sorbitol Dehydrogenase Products

Product Documents for Sorbitol Dehydrogenase Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Sorbitol Dehydrogenase Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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