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SORBS1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-06079

Novus Biologicals, part of Bio-Techne
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NBP1-06079

Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunohistochemistry, Peptide ELISA

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

Product Specifications

Immunogen

Peptide with sequence C-SERNDWEPPDKKVD corresponding to internal region according to NP_006425.2, NP_056200.1, NP_001030126.1, NP_001030127.1, NP_001030128.1, NP_079267.1, NP_001030129.1.

Epitope

C-SERNDWEPPDKKVD

Specificity

This antibody is expected to recognise all reported isoforms (NP_006425.2 and NP_056200.1 and NP_001030126.1 and NP_001030127.1 and NP_001030128.1 and NP_079267.1 and NP_001030129.1)

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for SORBS1 Antibody

Immunohistochemistry: SORBS1 Antibody [NBP1-06079]

Immunohistochemistry: SORBS1 Antibody [NBP1-06079]

Immunohistochemistry: SORBS1 Antibody [NBP1-06079] - (3.75ug/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Immunohistochemistry: SORBS1 Antibody [NBP1-06079]

Immunohistochemistry: SORBS1 Antibody [NBP1-06079]

Immunohistochemistry: SORBS1 Antibody [NBP1-06079] - (3.75ug/ml) staining of paraffin embedded Human Pancreas. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Applications for SORBS1 Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:32000
Application Notes
WB: Preliminary experiments gave an approx. 65 kDa band in human adipose lysates after 0.3 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 76.6 kDa band according to NP_006425.2 and higher sizes for other isoforms. The 65 kDa band was successfully blocked by incubation with the immunizing peptide.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: SORBS1

SORBS1 plays a role in tyrosine phosphorylation of CBL by linking CBL to the insulin receptor. Required for insulin-stimulated glucose transport. Involved in formation of actin stress fibers and focal adhesions

Alternate Names

c-Cbl associated protein, c-Cbl-associated protein, DKFZp451C066, DKFZp586P1422, Fas-ligand associated factor 2, FLAF2, KIAA0894, KIAA1296CAPSH3D5SH3P12FLJ12406, Ponsin, R85FL, SH3 domain protein 5, SH3-domain protein 5 (ponsin), sh3p12, SORB1, sorbin and SH3 domain containing 1, sorbin and SH3 domain-containing protein 1

Entrez Gene IDs

10580 (Human); 20411 (Mouse)

Gene Symbol

SORBS1

Additional SORBS1 Products

Product Documents for SORBS1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for SORBS1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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