SOX2 Antibody (SOX2/3169R)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-07891
Recombinant Monoclonal Antibody
![Immunohistochemistry-Paraffin: SOX2 Antibody (SOX2/3169R) [NBP3-07891]](https://resources.bio-techne.com/images/products/SOX2-Antibody-SOX2-3169R-Immunohistochemistry-Paraffin-NBP3-07891-img0001.jpg "Immunohistochemistry-Paraffin: SOX2 Antibody (SOX2/3169R) [NBP3-07891]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Human, Mouse, Canine
Applications
ELISA, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # SOX2/3169R
Concentration
0.2 mg/ml
Product Specifications
Immunogen
Recombinant fragment (within aa176-305) of human SOX2 protein (exact sequence is proprietary) (Uniprot: P48431)
Localization
Nuclear
Marker
Embryonic Stem Cell Marker
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
34 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-08508)
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Scientific Data Images for SOX2 Antibody (SOX2/3169R)
Immunohistochemistry-Paraffin: SOX2 Antibody (SOX2/3169R) [NBP3-07891]
Immunohistochemistry-Paraffin: SOX2 Antibody (SOX2/3169R) [NBP3-07891] - Formalin-fixed, paraffin-embedded human Skin stained with SOX2 Recombinant Rabbit Monoclonal Antibody (SOX2/3169R).Applications for SOX2 Antibody (SOX2/3169R)
Application
Recommended Usage
ELISA
2-4 ug/ml
Immunohistochemistry-Paraffin
1-2 ug/ml
Western Blot
1-2 ug/ml
Application Notes
ELISA: Use Ab at 2-4ug/ml for coating. Order Ab without BSA.
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: SOX2
Long Name
Transcription Factor SOX2
Alternate Names
ANOP3, MCOPS3, MGC2413, SRY (sex determining region Y)-box 2, SRY-related HMG-box gene 2, transcription factor SOX2, transcription factor SOX-2
Gene Symbol
SOX2
UniProt
Additional SOX2 Products
Product Documents for SOX2 Antibody (SOX2/3169R)
Product Specific Notices for SOX2 Antibody (SOX2/3169R)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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